FastQCFastQC Report
Thu 26 May 2016
SRR1512449_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512449_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2319293
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC66140.28517311094372294No Hit
GTATCAACGCAGAGTACTTTTTTTT53930.23252775738123646No Hit
CCCATGTACTCTGCGTTGATACCAC46450.20027654979340687No Hit
GGTATCAACGCAGAGTACTTTTTTT33440.14418186921617923No Hit
TATCAACGCAGAGTACTTTTTTTTT31770.13698139907290713No Hit
GAGTACATGGGAAGCAGTGGTATCA27260.117535818027304No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAC9100.011.3077091
GTACTAA1501.0895747E-911.2134771
TAGGACC4350.010.4589384
AGGACGT12250.010.0587235
GGACGTG12000.010.031326
GTAGGAC11400.09.8109883
TCCTACA10850.09.784382
ATTGTGC1857.075869E-109.7101848
TGTAGGA12400.09.7079392
TAGGACG12950.09.6613934
GTATTAG1459.814539E-79.5530631
CCTACAC2700.09.4784133
CCTACAG11200.09.4784123
GAACCGT800.0046024029.4784126
GGACCGT900.00113736149.4784126
CTTACAC2600.09.4784123
GTCCTAG2207.4578566E-119.4445071
GTCTAAG2950.09.3911481
GTGTAGG3800.09.3734941
TTATACT3050.09.3230294
Produced by FastQC (version 0.11.5)