FastQCFastQC Report
Thu 26 May 2016
SRR1512449_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512449_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2319293
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC73290.3160014711379718No Hit
GTATCAACGCAGAGTACTTTTTTTT71570.30858541805627837No Hit
CCCATGTACTCTGCGTTGATACCAC54460.23481293652850244No Hit
GGTATCAACGCAGAGTACTTTTTTT48050.20717520382288915No Hit
TATCAACGCAGAGTACTTTTTTTTT46970.2025186123529886No Hit
GAGTACATGGGAAGCAGTGGTATCA31950.1377574976512239No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATCA550.003071029712.0899629
AGGACGT12200.011.371645
GGCGAGG6000.011.23739319
GGACGTG11950.011.2083276
TGGACCG1201.2718556E-711.0860525
GTAGGAC12750.010.8061063
TTAGGAC7300.010.8035533
TAGGACG13000.010.744714
TGGCGAG12000.010.68389918
GACGTGG6700.010.4917157
TGTAGGA13450.010.3143522
GTATTAA2500.010.2742641
GACAGTC1856.002665E-1110.2694657
TATGGCG13100.010.22306816
TAGGACT3350.010.2112394
GACGTGA6350.010.1724327
CGTGAAA7200.010.1589269
TAGGACC4400.010.1499974
ATGGCGA13650.09.81009117
ACGTGAA7450.09.6905168
Produced by FastQC (version 0.11.5)