FastQCFastQC Report
Thu 26 May 2016
SRR1512448_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512448_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1658246
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC51600.31117216625277555No Hit
GTATCAACGCAGAGTACTTTTTTTT48940.29513112047307816No Hit
GTCCTACAGTGGACATTTCTAAATT37230.22451433623238048No Hit
GTCCTAAAGTGTGTATTTCTCATTT35400.21347857917341576No Hit
CCCATGTACTCTGCGTTGATACCAC34940.210704563737829No Hit
CTGTAGGACGTGGAATATGGCAAGA32320.19490473669166095No Hit
GGTATCAACGCAGAGTACTTTTTTT30900.18634147165137138No Hit
CTTTAGGACGTGAAATATGGCGAGG29430.17747668319417023No Hit
TATCAACGCAGAGTACTTTTTTTTT28890.17422023029152492No Hit
GAGTACATGGGAAGCAGTGGTATCA21750.13116268635654782No Hit
GTCCTACAGTGTGCATTTCTCATTT20020.12072997613140632No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC6050.013.1584034
GCGCAGA801.8513634E-512.3924661
GGCGAGG8550.012.29551619
AGCACCG802.9380159E-511.8464795
CTTACAC1950.011.6642263
AGGACCT13450.011.203515
TGTACGA600.005982022711.0567132
GTACGAC600.005982022711.0567133
GTCTTAC1457.417839E-910.9395551
GGACCTG13300.010.9023246
GACGTGA12050.010.6175917
AGGACGT21750.010.5447275
ACGAAAT1355.869333E-810.53275712
TCCAACG909.827888E-510.52191418
TGTAGGA21500.010.4910212
TTAGGAC12650.010.4885833
ACGTGAA13350.010.4804998
TGGCGAG21000.010.41669518
GGACGTG21900.010.342686
GTCTTAA2153.6379788E-1210.144531
Produced by FastQC (version 0.11.5)