FastQCFastQC Report
Thu 26 May 2016
SRR1512448_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512448_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1658246
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT60430.3644212016793648No Hit
GTACATGGGAAGCAGTGGTATCAAC56420.3402390236430542No Hit
CCCATGTACTCTGCGTTGATACCAC43170.26033531816147903No Hit
GGTATCAACGCAGAGTACTTTTTTT41960.25303845147221826No Hit
TATCAACGCAGAGTACTTTTTTTTT41890.252616318688542No Hit
CTGTAGGACGTGGAATATGGCAAGA38000.2291577968528192No Hit
GTCCTAAAGTGTGTATTTCTCATTT36650.22101666459620586No Hit
GTCCTACAGTGGACATTTCTAAATT36090.21763960232679588No Hit
CTTTAGGACGTGAAATATGGCGAGG34090.20557866565033173No Hit
GAGTACATGGGAAGCAGTGGTATCA24550.14804799770359764No Hit
GTCCTACAGTGTGCATTTCTCATTT21210.1279062334539025No Hit
ACGCAGAGTACTTTTTTTTTTTTTT20860.12579556953552126No Hit
CTGAAGGACCTGGAATATGGCGAGA19200.115784992094056No Hit
GTACTTTTTTTTTTTTTTTTTTTTT19160.11554377336052674No Hit
CTGTAGGACCTGGAATATGGCGAGA16610.1001660790980349No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTGC500.00149980113.2997888
CCAACGA550.003078678212.08561319
CCTATAC1603.6379788E-1211.8769613
TGTCGAG1052.73345E-711.75746318
TCCAACG1157.1107934E-811.5608518
TAGGACC6250.011.402574
TTATACT2650.011.1157134
AGGACGT22950.011.0961845
CGTAAAA600.00587663111.08315859
AATGTCC7200.011.0831588
TCGAACT1201.2829514E-711.0784819
CTAGACT1658.0035534E-1110.941864
AAATGTC7250.010.87568957
GGACGTG22050.010.8563176
TGTCCAC7950.010.75454710
TTGGACA909.493304E-510.5579364
GACGTGG11500.010.49113757
ATGTCCA8000.010.4498349
GGCGAGG10400.010.408919
GTACTAA1105.9530485E-610.3769331
Produced by FastQC (version 0.11.5)