FastQCFastQC Report
Thu 26 May 2016
SRR1512447_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512447_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2164298
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT54990.2540777656311654No Hit
CTGTAGGACGTGGAATATGGCAAGA52010.24030886689356085No Hit
GTATCAACGCAGAGTACTTTTTTTT50400.23286996522660003No Hit
GTCCTAAAGTGTGTATTTCTCATTT49380.22815712069225216No Hit
CTTTAGGACGTGAAATATGGCGAGG42410.19595268304087513No Hit
GTCCTACAGTGTGCATTTCTCATTT30950.1430024885667316No Hit
GGTATCAACGCAGAGTACTTTTTTT29570.136626287137908No Hit
TATCAACGCAGAGTACTTTTTTTTT28910.13357679949803586No Hit
CTGTAGGACCTGGAATATGGCGAGA22380.10340535360657359No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACCG400.005362238714.2168295
TAGGACC10350.013.4613474
GTGTAGG3900.011.9216851
GGCGAGG15450.011.28496419
GGACGAG1105.123002E-711.2011386
GGTATCA13000.011.1099961
TATTGCG600.00593446111.0688316
TGTAGGA35350.010.9391162
AGGACCT19800.010.913935
GTCCTAC30600.010.8299841
TTAGGAC19200.010.8107153
GACGTGA16700.010.6697167
AGGACGT33150.010.63581855
GTAGGAC34750.010.5279553
GGACGTG32500.010.5277456
TCCTACA35450.010.42701152
GTCTAGG2001.0913936E-1110.3870851
GGACCTG19400.010.3572786
GTCTTAG2900.010.2335821
TTTAGAA9450.010.23012
Produced by FastQC (version 0.11.5)