FastQCFastQC Report
Thu 26 May 2016
SRR1512447_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512447_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2164298
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT65370.3020378894218818No Hit
CTGTAGGACGTGGAATATGGCAAGA57890.2674770295033309No Hit
GTCCTACAGTGGACATTTCTAAATT53750.24834842521686018No Hit
CTTTAGGACGTGAAATATGGCGAGG47010.2172066878036204No Hit
GTCCTAAAGTGTGTATTTCTCATTT46320.2140185870892086No Hit
TATCAACGCAGAGTACTTTTTTTTT45150.20861267718216256No Hit
GGTATCAACGCAGAGTACTTTTTTT44440.205332167751391No Hit
GTCCTACAGTGTGCATTTCTCATTT29150.134685704094353No Hit
CTGTAGGACCTGGAATATGGCGAGA27050.12498278887657799No Hit
CTGAAGGACCTGGAATATGGCGAGA26900.12428972350387979No Hit
ACGCAGAGTACTTTTTTTTTTTTTT21820.10081790954850026No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC9850.012.9262644
CCAACGA951.0444455E-611.99613319
TGTAGGA33000.011.3437492
GGTATCA15600.011.3413521
TAGCGAG855.3398613E-511.17338618
AAATCGT600.00587990211.0825796
TATAGCG951.3635399E-510.9979816
GTAGGAC32800.010.862553
AGGACCT21550.010.8027245
CTGTAGG32650.010.7794111
GTAGAAC4500.010.7679243
GGCGAGG14850.010.68005919
GTCCTAA15300.010.6311731
GACGTGG15750.010.615157
GTATTAG1803.45608E-1110.5690031
CCCGTTT1355.6958015E-810.55483712
TAGAACA6350.010.4743914
CTACACT4450.010.4626224
ATAGCGA1106.0642105E-610.36125517
GGACCTG21300.010.3467136
Produced by FastQC (version 0.11.5)