FastQCFastQC Report
Thu 26 May 2016
SRR1512446_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512446_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2637966
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT58180.22054871063539103No Hit
GGTATCAACGCAGAGTACTTTTTTT34140.12941789242166124No Hit
TATCAACGCAGAGTACTTTTTTTTT33840.1282806525937029No Hit
GTCCTACAGTGGACATTTCTAAATT28790.10913711548973717No Hit
CTGTAGGACGTGGAATATGGCAAGA26690.10117643669402866No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG2100.011.2874971
TAGGACC5250.011.0130754
GTCTAGT1156.465145E-610.3059761
ACCGTGC856.875557E-410.0135268
GTCCTAA9050.09.9311271
TGTAGGA17550.09.6676792
GTATCAA31900.09.5979111
GTCCTAC17300.09.591111
GTAGGAC16300.09.5366163
GTATTGA2600.09.4966921
GGACCGC1002.8199927E-49.4784676
TAATACT3350.09.3369964
AGGACGT15400.09.293825
TCCTACA19750.09.2626772
GTATTGG3100.09.2393621
GGTATCA17450.09.225671
GTATAGG2800.09.1710921
CCGTGCA1451.7660641E-69.1495329
GGACGTG15150.09.1343646
AGGACCT12900.09.1110845
Produced by FastQC (version 0.11.5)