FastQCFastQC Report
Thu 26 May 2016
SRR1512446_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512446_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2637966
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT71930.27267220275014914No Hit
GGTATCAACGCAGAGTACTTTTTTT48370.18336096826115272No Hit
TATCAACGCAGAGTACTTTTTTTTT48190.1826786243643777No Hit
CTGTAGGACGTGGAATATGGCAAGA30460.11546775053203871No Hit
GTCCTACAGTGGACATTTCTAAATT30190.11444423468687619No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACCGT752.0762454E-411.3991816
TTAGGAC10200.011.2709353
TAGGACC6950.011.2101664
GTCGACT600.005881472511.0823278
GGCGAGG7300.010.9278219
GTCTACG700.001472706610.8740941
GACGTGA8100.010.2027777
GGTATCA21450.010.2023841
TGTAGGA19000.010.2012012
TAGGACA6550.010.1540594
TGGCGAG17400.010.1519418
AGGACGT15250.010.031075
GGACGTG15250.010.0287886
GTAGGAC18350.09.9930033
TAGGACT4900.09.8891084
GAAGCGT1356.453447E-79.8511446
AAGCGTG1554.0723535E-89.8055617
CTAATAC2350.09.703273
GTCCTAC15900.09.6943571
AGGACCT13350.09.679415
Produced by FastQC (version 0.11.5)