FastQCFastQC Report
Thu 26 May 2016
SRR1512445_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512445_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2044172
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTAAAGTGTGTATTTCTCATTT61990.30325236819602264No Hit
GTCCTACAGTGGACATTTCTAAATT61430.30051287269368726No Hit
CTGTAGGACGTGGAATATGGCAAGA55510.27155249166899853No Hit
CTTTAGGACGTGAAATATGGCGAGG50270.24591864089714566No Hit
GTATCAACGCAGAGTACTTTTTTTT48300.23628148707642996No Hit
GGTATCAACGCAGAGTACTTTTTTT31620.15468365675686782No Hit
GTCCTACAGTGTGCATTTCTCATTT31180.15253119600503284No Hit
TATCAACGCAGAGTACTTTTTTTTT29610.14485082468598534No Hit
GTACATGGGAAGCAGTGGTATCAAC29600.14480190512344362No Hit
CTGAAGGACCTGGAATATGGCGAGA25920.1267995061080966No Hit
ATTTAGAAATGTCCACTGTAGGACG24410.11941265216429929No Hit
CTGTAGGACCTGGAATATGGCGAGA23160.1132977068465863No Hit
GTCCTTCAGTGTGCATTTCTCATTT22050.10786763540445717No Hit
TTTCTAAATTTTCCACCTTTTTCAG21220.10380731171349573No Hit
GAATATGGCAAGAAAACTGAAAATC20480.10018726408540964No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAACG1400.013.52890918
TCGAACT1501.8189894E-1212.62884219
ACCGTGC550.003179721812.0338198
TAGGACC9700.012.0175054
ATTAGAC951.0708882E-611.9712233
CCAACGA1157.3769115E-811.53068319
CCACCTT7900.011.28362413
GACCGTG600.00598266711.0567547
AATGTCC10000.010.9679678
GTGTAGG3800.010.9548411
ACCTTTT8600.010.81184415
GGACCTG18650.010.7222156
TTTAGAA11600.010.7027212
CACCTTT8600.010.70151914
AAGTCGT3100.010.7000867
AGGACCT19400.010.698515
CTCGAAC1605.7480065E-1010.654016518
GAAATGT10150.010.6443636
GGCGAGG14550.010.61083219
AGTCGTC3050.010.540168
Produced by FastQC (version 0.11.5)