FastQCFastQC Report
Thu 26 May 2016
SRR1512445_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512445_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2044172
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGA64280.31445494801807283No Hit
GTCCTAAAGTGTGTATTTCTCATTT63650.3113730155779455No Hit
GTATCAACGCAGAGTACTTTTTTTT62960.3079975657625679No Hit
GTCCTACAGTGGACATTTCTAAATT62500.30574726588564954No Hit
CTTTAGGACGTGAAATATGGCGAGG60510.29601227293985044No Hit
TATCAACGCAGAGTACTTTTTTTTT44030.21539283387112237No Hit
GGTATCAACGCAGAGTACTTTTTTT42580.20829949730257533No Hit
GTCCTACAGTGTGCATTTCTCATTT33930.16598407570400142No Hit
GTACATGGGAAGCAGTGGTATCAAC32790.16040724557424718No Hit
CTGAAGGACCTGGAATATGGCGAGA32620.1595756130110382No Hit
ATTTAGAAATGTCCACTGTAGGACG29330.14348107693481763No Hit
CTGTAGGACCTGGAATATGGCGAGA28300.13844236199302212No Hit
TTTCTAAATTTTCCACCTTTTTCAG26150.12792465604655576No Hit
CCCATGTACTCTGCGTTGATACCAC24760.12112483685325892No Hit
GAATATGGCAAGAAAACTGAAAATC24450.1196083304144661No Hit
GTCCTTCAGTGTGCATTTCTCATTT23170.113346626409128No Hit
ACGCAGAGTACTTTTTTTTTTTTTT21120.1033181160880787No Hit
GGAATATGGCGAGAAAACTGAAAAT21050.10297567915028677No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCGTC307.727747E-418.998588
TAGGACC11200.012.7256894
CGTGTAA658.0303516E-411.6914349
AAATGTC10300.011.5282657
AATGTCC10450.011.453698
GACCTAC752.0486637E-411.4153471
ACCGAGC600.005879999611.0825058
TAGAAAT11150.011.0783884
ACCTTTT10700.011.00744615
TACGGTG700.001488723910.8597865
TTCCACC10450.010.72647111
ATGTCCA11350.010.7128559
CCACCTT11000.010.7080313
TCCAACG2050.010.65565518
CACCTTT11200.010.60084914
GAAATGT11400.010.5822846
GGCGAGG15350.010.57994219
TGGCGAG35800.010.55859818
AGGACCT23950.010.4743665
TGTCCAC11700.010.39238610
Produced by FastQC (version 0.11.5)