FastQCFastQC Report
Thu 26 May 2016
SRR1512444_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512444_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences873390
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT35750.4093245858093177No Hit
GGTATCAACGCAGAGTACTTTTTTT23840.27295938813130444No Hit
TATCAACGCAGAGTACTTTTTTTTT21420.24525126232267372No Hit
GTACATGGGAAGCAGTGGTATCAAC18930.2167416618005702No Hit
CCCATGTACTCTGCGTTGATACCAC13640.15617307273955505No Hit
GTACTTTTTTTTTTTTTTTTTTTTT11400.1305258819084258No Hit
ACGCAGAGTACTTTTTTTTTTTTTT10720.12274012754897583No Hit
GAGTACATGGGAAGCAGTGGTATCA8800.1007568211222936No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGGT250.00611381518.9546364
GGACTAT905.554466E-712.6364226
GTAGGAC4450.012.3524583
TAGGACC1551.8189894E-1212.2287964
CTTACTA550.003130043412.057889
GAGGTGT951.0684143E-611.9713486
TATACTC600.0059775411.056875
GACTATC600.0059775411.056877
GACGTGG2100.010.831227
TATGGAC700.001520271910.831223
TGTAGGA5300.010.7290382
TTTATAC1159.072701E-710.713493
GACTTAC650.007777119510.6720791
CTAAGCT803.8512037E-410.6619824
GAGTGCC909.736197E-510.5303527
GCGCAGA854.4639246E-410.4927161
GTGTAAA951.0712678E-410.4313551
TGGCGAG4000.010.41965718
AGGACGT4100.010.4019335
GTCCTAC4100.010.3931911
Produced by FastQC (version 0.11.5)