FastQCFastQC Report
Thu 26 May 2016
SRR1512444_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512444_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences873390
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT45230.5178671612910613No Hit
GGTATCAACGCAGAGTACTTTTTTT33320.381501963613048No Hit
TATCAACGCAGAGTACTTTTTTTTT32740.3748611731299878No Hit
GTACATGGGAAGCAGTGGTATCAAC21910.25086158531698327No Hit
ACGCAGAGTACTTTTTTTTTTTTTT15950.18262173828415715No Hit
CCCATGTACTCTGCGTTGATACCAC14460.1655617765259506No Hit
GTACTTTTTTTTTTTTTTTTTTTTT13430.15376864859913672No Hit
GAGTACTTTTTTTTTTTTTTTTTTT10970.12560253723995007No Hit
GAGTACATGGGAAGCAGTGGTATCA9900.11335142376258028No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTAGT853.876159E-612.3114541
GTGTAGG701.0755996E-412.2315081
CTTATGG701.0871781E-412.217493
TAGTCCC550.003069780512.0892329
GGGTAGG951.0205058E-612.0169211
ATAATAG802.85531E-511.8781163
TATACTA752.0645873E-411.4042985
TAGGACC2000.011.40364554
TAAGACC752.0656867E-411.4036454
CTTACAC752.0667867E-411.4029913
AGGACCT4050.010.7941925
GCCTAGA803.7183618E-410.702571
GTAGGAG909.4779476E-510.5583253
GGACCAT909.5220996E-510.5534876
GTATTAT1002.3581018E-510.4647351
GTATAAT1002.3581018E-510.4647351
TTATACT1002.3866316E-510.45334154
GGACTGT1002.400254E-510.4479526
TATACAC2350.010.1090353
GTTCTAG856.501978E-410.0730081
Produced by FastQC (version 0.11.5)