##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512444_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 873390 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.86783338485671 31.0 31.0 34.0 28.0 34.0 2 31.165913280436 31.0 31.0 34.0 28.0 34.0 3 31.315881793929403 33.0 31.0 34.0 28.0 34.0 4 34.8834976356496 37.0 35.0 37.0 32.0 37.0 5 34.23030261395253 37.0 35.0 37.0 30.0 37.0 6 34.49189594568291 37.0 35.0 37.0 31.0 37.0 7 34.43131590698314 37.0 35.0 37.0 31.0 37.0 8 34.40904864951511 37.0 35.0 37.0 31.0 37.0 9 35.89309472286149 39.0 37.0 39.0 30.0 39.0 10 35.806420957418794 39.0 37.0 39.0 30.0 39.0 11 35.8559818637722 39.0 37.0 39.0 30.0 39.0 12 35.74544590618166 39.0 37.0 39.0 30.0 39.0 13 35.70158920986043 39.0 37.0 39.0 30.0 39.0 14 36.85087417991962 40.0 37.0 41.0 30.0 41.0 15 36.81769198181797 40.0 37.0 41.0 30.0 41.0 16 36.775296259403014 40.0 37.0 41.0 30.0 41.0 17 36.7448230458329 40.0 37.0 41.0 30.0 41.0 18 36.67837277733887 40.0 37.0 41.0 30.0 41.0 19 36.70700374403187 40.0 37.0 41.0 30.0 41.0 20 36.68159470568703 40.0 37.0 41.0 30.0 41.0 21 36.61465095776228 40.0 37.0 41.0 30.0 41.0 22 36.53268413881542 40.0 37.0 41.0 30.0 41.0 23 36.403401687676755 40.0 37.0 41.0 30.0 41.0 24 36.3406771316365 40.0 37.0 41.0 30.0 41.0 25 36.259519802150244 40.0 37.0 41.0 30.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.4196808081865555 1101 2 0.6120547212828207 1101 3 0.5574788775466288 1101 4 0.5196752098979971 1101 5 0.26082446137808546 1101 6 0.5688077136440413 1101 7 0.5116492751077502 1101 8 0.40036849925692053 1101 9 0.6697995999201751 1101 10 0.6200283345553075 1101 11 0.6600333476130018 1101 12 0.3720747962992661 1101 13 0.7429804667691542 1101 14 0.7249130471764076 1101 15 0.68887627180181 1101 16 0.7578856392673927 1101 17 0.5907782292712511 1101 18 0.5961879976728142 1101 19 0.8103184376374557 1101 20 0.7608275582066497 1101 21 0.48179713595820317 1101 22 0.6226199319120695 1101 23 0.7406577628619715 1101 24 0.5266382692645308 1101 25 0.735215501773034 1102 1 0.2058400875802313 1102 2 0.30611448081617354 1102 3 0.3639680380296788 1102 4 0.5438409176080086 1102 5 0.13732407118192924 1102 6 0.35009946132601044 1102 7 0.5493065109317214 1102 8 0.6828969703493399 1102 9 0.5882419897724276 1102 10 0.6793910582567477 1102 11 0.4975530227581686 1102 12 0.8198612597993957 1102 13 0.9489246753323073 1102 14 1.0180627899114825 1102 15 1.235613835126017 1102 16 1.0170287949622718 1102 17 1.0106425203708085 1102 18 1.0373212860343912 1102 19 1.2361216465048273 1102 20 1.0772171194674485 1102 21 1.0753846920075674 1102 22 1.2092633190792412 1102 23 0.9952376075771241 1102 24 0.9890537818764642 1102 25 0.8837016729567111 1103 1 0.08923332893400371 1103 2 0.1396574390167622 1103 3 0.03743101955502581 1103 4 0.09540544269595586 1103 5 -0.3854037335881557 1103 6 -0.038791910429324616 1103 7 0.17244462711710185 1103 8 0.26947736095389274 1103 9 0.4072357052827513 1103 10 0.3742815319238204 1103 11 0.586727654105708 1103 12 0.53979924488943 1103 13 0.5408074045842355 1103 14 0.4669111300443234 1103 15 0.5506119091310424 1103 16 0.3091724380083676 1103 17 0.29235599616392705 1103 18 0.3808528159554143 1103 19 0.43018958198719304 1103 20 0.40143443128922485 1103 21 0.5072108544679068 1103 22 0.6381749510247943 1103 23 0.6050523077071617 1103 24 0.5964178928222879 1103 25 0.6864680827531089 1104 1 0.17890187001091462 1104 2 0.21963156446988563 1104 3 0.21314950790080545 1104 4 0.19935835614431596 1104 5 -0.18108447754542567 1104 6 0.15998096997134326 1104 7 0.3145155302071032 1104 8 0.32687743866292607 1104 9 0.43156461090897125 1104 10 0.4388398072956079 1104 11 0.3834352674562922 1104 12 0.48632091162014035 1104 13 0.30009085896433874 1104 14 0.44329099913449 1104 15 0.41192476485269225 1104 16 0.5027874879428254 1104 17 0.38205015560407674 1104 18 0.6004640999904751 1104 19 0.5368879300297351 1104 20 0.5105024779317233 1104 21 0.5472438167619416 1104 22 0.7808115929697408 1104 23 0.6600378546805601 1104 24 0.5561693093042948 1104 25 0.7133054531135201 1105 1 0.18593897909042667 1105 2 0.3643829098631315 1105 3 0.28645993172669293 1105 4 0.3047593805883295 1105 5 0.19467899259657173 1105 6 0.4610443169207059 1105 7 0.4544191373738897 1105 8 0.5163520328836739 1105 9 0.5855316298633468 1105 10 0.5447919566828858 1105 11 0.4698170781718858 1105 12 0.4664479945482327 1105 13 0.5285937442655069 1105 14 0.7350111573397626 1105 15 0.5625526150331623 1105 16 0.5979981041221265 1105 17 0.6765540350914208 1105 18 0.6149511215383825 1105 19 0.746739018231338 1105 20 0.651236014424839 1105 21 0.8317431575732854 1105 22 0.5672215002510725 1105 23 0.6629858328084666 1105 24 0.728790286632524 1105 25 0.769355940480871 1106 1 0.2816337140326155 1106 2 0.32261493763766325 1106 3 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0.5476326544052768 1107 16 0.6064009430590787 1107 17 0.5810714676148692 1107 18 0.538475962236717 1107 19 0.5083563834077367 1107 20 0.5648349368721313 1107 21 0.4630439916405322 1107 22 0.6187212671266877 1107 23 0.699311792503849 1107 24 0.3940547532957339 1107 25 0.5253109733773087 1108 1 0.0700427369886647 1108 2 0.06897220151594041 1108 3 0.1543681458936348 1108 4 0.164564280266859 1108 5 -0.12058539050951111 1108 6 0.09165067985164654 1108 7 -0.0032906508979166915 1108 8 0.055172562758414756 1108 9 0.2439065328674488 1108 10 0.28651682088862884 1108 11 0.29452609047650924 1108 12 0.2168747765014558 1108 13 0.4909815061544336 1108 14 0.5610836548455822 1108 15 0.5719639884950851 1108 16 0.5317859325225243 1108 17 0.6298962366211853 1108 18 0.5718849426225177 1108 19 0.3462069264455039 1108 20 0.5387096869666905 1108 21 0.5865486115140897 1108 22 0.6493853401801317 1108 23 0.5887590286847555 1108 24 0.39792896305420555 1108 25 0.6869038841678261 1109 1 -0.18373780556247965 1109 2 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19 -1.067870790576528 2314 20 -1.227085548201181 2314 21 -1.0186120317058922 2314 22 -1.0676614992629325 2314 23 -0.8485529071226665 2314 24 -0.9845766182057005 2314 25 -0.8243157963791958 2315 1 -0.8877035661248307 2315 2 -1.0288751810260202 2315 3 -0.9545917343085222 2315 4 -1.0112848077304832 2315 5 -0.6110140421598516 2315 6 -1.0980874703532137 2315 7 -1.2594375696392106 2315 8 -1.6354515923132382 2315 9 -1.6895765477673095 2315 10 -1.7363362811683132 2315 11 -1.5614856397287653 2315 12 -1.6030249759995314 2315 13 -1.7586722032110131 2315 14 -1.686454873887179 2315 15 -2.066271489136412 2315 16 -2.029348403285802 2315 17 -1.9551415121777111 2315 18 -1.789998129692954 2315 19 -1.930444973473385 2315 20 -1.7433102377436995 2315 21 -1.5941277471306918 2315 22 -1.3632038322580513 2315 23 -1.5940262711218196 2315 24 -1.5693512515835906 2315 25 -1.659185007494166 2316 1 -0.6752704934928673 2316 2 -0.4722094254125295 2316 3 -0.7762828592489548 2316 4 -0.7458494644736788 2316 5 -0.49825018714345504 2316 6 -0.6106985487598564 2316 7 -1.134987705596373 2316 8 -0.8791600693126469 2316 9 -0.9352718215037541 2316 10 -0.9883546143614836 2316 11 -0.9711529651569606 2316 12 -0.980250048182981 2316 13 -0.8713782413911488 2316 14 -1.2235294821343103 2316 15 -1.1546313831739212 2316 16 -1.313837022129185 2316 17 -1.260934759171235 2316 18 -1.2839413780880946 2316 19 -1.4392594129654341 2316 20 -1.4122370171006366 2316 21 -1.442302487130135 2316 22 -1.4162761838965423 2316 23 -1.3614221307355336 2316 24 -1.3554955343744979 2316 25 -1.2264960690813567 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 13455.0 3 1.0 4 291.0 5 2201.0 6 2430.0 7 1873.0 8 1552.0 9 1442.0 10 1384.0 11 1244.0 12 1171.0 13 1136.0 14 1130.0 15 1103.0 16 1130.0 17 1231.0 18 1226.0 19 1394.0 20 1799.0 21 2435.0 22 3119.0 23 3797.0 24 4474.0 25 5238.0 26 6085.0 27 6810.0 28 7926.0 29 9498.0 30 11842.0 31 15625.0 32 20239.0 33 26743.0 34 35514.0 35 49122.0 36 73970.0 37 150053.0 38 403707.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.512562929271624 19.732051975681102 13.828506845420948 23.92687824962632 2 16.390704022478523 21.528979226373334 38.30921021746872 23.771106533679426 3 19.229611038532042 25.338198738918017 30.483857486514985 24.948332736034953 4 13.669459319893331 17.800387696753813 36.61100825200976 31.919144731343096 5 13.551243973705942 37.91665759882893 35.047402837641414 13.484695589823708 6 32.17720527438095 36.740296059789145 17.52355125160043 13.558947414229468 7 27.745948712898272 32.08346836669373 22.051300843638813 18.119282076769185 8 26.078198984294932 35.32901630052045 20.48678266545286 18.10600204973176 9 26.90172457229233 14.649817923642445 19.89636534365481 38.55209216041042 10 15.616650767456589 28.734357983025827 33.89406201722612 21.754929232291467 11 34.155928041668766 22.474266238495964 24.040166198641273 19.329639521193993 12 23.27928105391065 25.319097034129783 30.96589770341756 20.43572420854201 13 29.10398961054429 21.644470351915327 25.78582283641761 23.465717201122775 14 21.60743485569546 21.99871291618382 27.487140612761824 28.906711615358894 15 23.889689858653966 29.55234887723634 23.696159276814004 22.861801987295692 16 22.55625752209248 27.78642353202536 27.139652406325254 22.517666539556902 17 22.10180733032016 28.213728290107266 27.520133932303263 22.164330447269307 18 22.111418750100192 26.802378973065938 29.290247080673936 21.795955196159934 19 23.357385923853055 26.800967294278216 28.312593603583387 21.52905317828534 20 23.658463698056867 26.662643994673584 28.278315469845417 21.400576837424133 21 23.34291671389659 26.174765596465257 28.00156173609179 22.480755953546367 22 23.38749937025112 26.91306259474858 27.67024974695545 22.02918828804485 23 22.66951845206676 26.778196207568143 28.085658900971065 22.466626439394027 24 22.90870660921876 26.77350175355032 28.320496100740694 21.99729553649022 25 22.76041946944603 26.634065691232657 28.045349781139905 22.560165058181404 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 568.0 1 568.0 2 565.5 3 563.0 4 563.0 5 563.0 6 1208.0 7 1853.0 8 1853.0 9 1853.0 10 2253.5 11 2654.0 12 2654.0 13 2654.0 14 3502.5 15 4351.0 16 4351.0 17 4351.0 18 7582.5 19 10814.0 20 10814.0 21 10814.0 22 16428.5 23 22043.0 24 22043.0 25 22043.0 26 30985.5 27 39928.0 28 39928.0 29 39928.0 30 49751.0 31 59574.0 32 59574.0 33 59574.0 34 67626.5 35 75679.0 36 75679.0 37 75679.0 38 82381.0 39 89083.0 40 89083.0 41 89083.0 42 95204.0 43 101325.0 44 101325.0 45 101325.0 46 109062.0 47 116799.0 48 116799.0 49 116799.0 50 113896.0 51 110993.0 52 110993.0 53 110993.0 54 100729.5 55 90466.0 56 90466.0 57 90466.0 58 77433.5 59 64401.0 60 64401.0 61 64401.0 62 53193.5 63 41986.0 64 41986.0 65 41986.0 66 32550.5 67 23115.0 68 23115.0 69 23115.0 70 16953.5 71 10792.0 72 10792.0 73 10792.0 74 7450.5 75 4109.0 76 4109.0 77 4109.0 78 2849.0 79 1589.0 80 1589.0 81 1589.0 82 1046.5 83 504.0 84 504.0 85 504.0 86 326.5 87 149.0 88 149.0 89 149.0 90 97.0 91 45.0 92 45.0 93 45.0 94 26.0 95 7.0 96 7.0 97 7.0 98 3.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.11243545266146854 2 0.0026334169156963095 3 0.0014884530393066099 4 0.0030914024662521897 5 0.03904326818488876 6 0.02152532087612636 7 0.017059961758206527 8 0.012022120702091849 9 0.014884530393066099 10 0.02072384616265357 11 0.024960212505295457 12 0.025532694443490305 13 0.02255578836487709 14 0.01122064598861906 15 0.016372983432372708 16 0.014770034005427129 17 0.012938091803203608 18 0.00824373991000584 19 0.0029769060786132197 20 8.0147471347279E-4 21 3.4348916291691E-4 22 0.0029769060786132197 23 0.00217543136514043 24 0.00217543136514043 25 1.1449638763896998E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 873390.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 75.49690708399389 #Duplication Level Percentage of deduplicated Percentage of total 1 85.99644688679771 64.92465760166183 2 10.104833208851606 15.257673077358511 3 2.10162169387445 4.759978132444358 4 0.7480391218989415 2.2589856032478703 5 0.32406277671213546 1.2232868671408577 6 0.18786518973415012 0.850994446420561 7 0.12112372938894139 0.6401126860340609 8 0.08399062541394423 0.5072825954242468 9 0.05026772973395625 0.3415552308943031 >10 0.25061670485219 3.3623354699483263 >50 0.015947684027206137 0.8181140616243985 >100 0.012147719108942247 1.9822298537528746 >500 0.001822157763509091 0.9174590872819031 >1k 0.0012147718423393939 2.1553352867658453 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 4523 0.5178671612910613 No Hit GGTATCAACGCAGAGTACTTTTTTT 3332 0.381501963613048 No Hit TATCAACGCAGAGTACTTTTTTTTT 3274 0.3748611731299878 No Hit GTACATGGGAAGCAGTGGTATCAAC 2191 0.25086158531698327 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 1595 0.18262173828415715 No Hit CCCATGTACTCTGCGTTGATACCAC 1446 0.1655617765259506 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 1343 0.15376864859913672 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 1097 0.12560253723995007 No Hit GAGTACATGGGAAGCAGTGGTATCA 990 0.11335142376258028 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 1.1449638763896999E-4 0.0 4 0.0 0.0 0.0 1.1449638763896999E-4 0.0 5 0.0 0.0 0.0 1.1449638763896999E-4 0.0 6 0.0 0.0 0.0 1.1449638763896999E-4 0.0 7 0.0 0.0 0.0 1.1449638763896999E-4 0.0 8 0.0 0.0 0.0 1.1449638763896999E-4 0.0 9 0.0 0.0 0.0 1.1449638763896999E-4 0.0 10 0.0 0.0 0.0 1.1449638763896999E-4 0.0 11 0.0 0.0 0.0 1.1449638763896999E-4 0.0 12 0.0 0.0 0.0 1.1449638763896999E-4 0.0 13 0.0 0.0 0.0 2.2899277527793998E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCTAGT 85 3.876159E-6 12.311454 1 GTGTAGG 70 1.0755996E-4 12.231508 1 CTTATGG 70 1.0871781E-4 12.21749 3 TAGTCCC 55 0.0030697805 12.089232 9 GGGTAGG 95 1.0205058E-6 12.016921 1 ATAATAG 80 2.85531E-5 11.878116 3 TATACTA 75 2.0645873E-4 11.404298 5 TAGGACC 200 0.0 11.4036455 4 TAAGACC 75 2.0656867E-4 11.403645 4 CTTACAC 75 2.0667867E-4 11.402991 3 AGGACCT 405 0.0 10.794192 5 GCCTAGA 80 3.7183618E-4 10.70257 1 GTAGGAG 90 9.4779476E-5 10.558325 3 GGACCAT 90 9.5220996E-5 10.553487 6 GTATTAT 100 2.3581018E-5 10.464735 1 GTATAAT 100 2.3581018E-5 10.464735 1 TTATACT 100 2.3866316E-5 10.4533415 4 GGACTGT 100 2.400254E-5 10.447952 6 TATACAC 235 0.0 10.109035 3 GTTCTAG 85 6.501978E-4 10.073008 1 >>END_MODULE