FastQCFastQC Report
Thu 26 May 2016
SRR1512443_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512443_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2445924
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT73900.30213530755657164No Hit
GTATCAACGCAGAGTACTTTTTTTT73490.3004590494226313No Hit
GTCCTAAAGTGTGTATTTCTCATTT68480.2799759927127744No Hit
CTGTAGGACGTGGAATATGGCAAGA66560.27212619852456577No Hit
CTTTAGGACGTGAAATATGGCGAGG57670.2357800160593706No Hit
GGTATCAACGCAGAGTACTTTTTTT47410.19383267836613075No Hit
TATCAACGCAGAGTACTTTTTTTTT44390.18148560625759425No Hit
GTCCTACAGTGTGCATTTCTCATTT44210.1807496880524497No Hit
CTGAAGGACCTGGAATATGGCGAGA29880.12216242205399677No Hit
CTGTAGGACCTGGAATATGGCGAGA28170.11517119910512347No Hit
GTCCTTCAGTGTGCATTTCTCATTT26320.10760759533002662No Hit
ATTTAGAAATGTCCACTGTAGGACG25250.1032329704438895No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15350.012.9977171
CCAACGA1550.012.8376819
TAGGACC11700.012.8066684
GCACCGT604.1528273E-412.6450786
GACCGTG701.1086925E-412.1937187
TCCAACG2050.012.01416418
GGCGAGG18050.011.96896519
GTGGTAT5000.011.4572941
GTGTAGG6000.011.0292921
AGGACGT42750.011.0029845
GTCCTAT3550.010.8507641
GGACGTG43250.010.8323736
TTAGGAC24700.010.7888283
GGACCTG21900.010.7396556
GACGTGG23150.010.7335157
AGGACCT23450.010.6764285
AATGTCC10100.010.6696148
TGTAGGA42500.010.6660572
CCGCAGA650.00797814910.6367451
TGTACGA909.6893025E-510.5371312
Produced by FastQC (version 0.11.5)