FastQCFastQC Report
Thu 26 May 2016
SRR1512443_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512443_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2445924
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGA80180.32781067604717073No Hit
GTATCAACGCAGAGTACTTTTTTTT79940.32682945177364464No Hit
GTCCTACAGTGGACATTTCTAAATT75760.3097397956763988No Hit
GTCCTAAAGTGTGTATTTCTCATTT71600.29273190826861345No Hit
CTTTAGGACGTGAAATATGGCGAGG69410.28377823677268793No Hit
GGTATCAACGCAGAGTACTTTTTTT57380.23459437006219325No Hit
TATCAACGCAGAGTACTTTTTTTTT55130.2253953924978863No Hit
GTCCTACAGTGTGCATTTCTCATTT45610.18647349631468516No Hit
CTGAAGGACCTGGAATATGGCGAGA37190.15204887805181191No Hit
CTGTAGGACCTGGAATATGGCGAGA34750.14207309793763012No Hit
ATTTAGAAATGTCCACTGTAGGACG28630.11705187896271511No Hit
TTTCTAAATTTTCCACCTTTTTCAG28460.11635684510230081No Hit
GTCCTTCAGTGTGCATTTCTCATTT26590.10871147263774344No Hit
ACGCAGAGTACTTTTTTTTTTTTTT25930.10601310588554673No Hit
GAATATGGCAAGAAAACTGAAAATC25740.10523630333567192No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGTCC400.005284964614.2480839
GGTATCA17400.013.6161131
TAGGACC12800.012.8494174
ACCGGGG1603.6379788E-1211.86903215
GGCGAGG18150.011.82253219
GGCGAAC1950.011.69073510
GTAGAAC6000.011.5669513
CCACCTT10100.011.47159213
TGGTATC4950.011.3319062
CGCCCTA2450.011.23855716
GTGGTAT5450.011.173431
GCGAACC1900.010.9985211
ACCTTTT10450.010.994472515
AGGACGT43550.010.7848175
GGACGTG42750.010.7763286
CCAACGA1158.886782E-710.73307619
TAGAAAT10900.010.7281774
TCCAACG2050.010.65252718
TGTAGGA43800.010.6359952
AGGACCT26300.010.6283445
Produced by FastQC (version 0.11.5)