FastQCFastQC Report
Thu 26 May 2016
SRR1512442_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512442_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2182119
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT65690.30103766109914265No Hit
GTCCTACAGTGGACATTTCTAAATT60500.27725344034857863No Hit
GTCCTAAAGTGTGTATTTCTCATTT54900.25159031198573495No Hit
CTGTAGGACGTGGAATATGGCAAGA53370.24457877870088662No Hit
CTTTAGGACGTGAAATATGGCGAGG47670.2184573801887065No Hit
GGTATCAACGCAGAGTACTTTTTTT41510.19022793898957846No Hit
TATCAACGCAGAGTACTTTTTTTTT37860.17350107853879645No Hit
GTCCTACAGTGTGCATTTCTCATTT35610.16319000017872537No Hit
CTGTAGGACCTGGAATATGGCGAGA26190.12002095211122767No Hit
CTGAAGGACCTGGAATATGGCGAGA23960.10980152778102385No Hit
ATTTAGAAATGTCCACTGTAGGACG22140.10146101106309968No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11350.013.7371741
CGTGCGC1153.9108272E-1013.19006910
GCGTGCG1207.9489837E-1012.6099839
GGCGTGC1208.0035534E-1012.605068
TAGGACC10650.012.0223464
GCGCCAC1052.7756505E-711.74408413
TCAATAC2200.011.6398163
AGGCGTG1850.011.2786227
TGTCGAG1604.7293724E-1111.24963818
GGCGAGG15100.011.23184219
TCCAACG2200.011.19581218
GTCCTAA15800.011.1172951
GGACGTA600.005949744511.065016
GTCCTAC30600.010.8678761
ATGTCGA1502.1791493E-910.7530817
GATATAC5600.010.7492471
CCACCTT7550.010.6791613
ACGAAAT2050.010.6377312
TGGCGAG34300.010.63338618
TAGGACA10550.010.6080274
Produced by FastQC (version 0.11.5)