FastQCFastQC Report
Thu 26 May 2016
SRR1512442_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512442_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2182119
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT83050.3805933590239579No Hit
GTCCTACAGTGGACATTTCTAAATT63280.28999335050013314No Hit
CTGTAGGACGTGGAATATGGCAAGA61680.2826610281107492No Hit
GTCCTAAAGTGTGTATTTCTCATTT58500.2680880373618487No Hit
GGTATCAACGCAGAGTACTTTTTTT56370.2583268831809814No Hit
TATCAACGCAGAGTACTTTTTTTTT53910.24705343750730369No Hit
CTTTAGGACGTGAAATATGGCGAGG52940.2426082170587397No Hit
GTCCTACAGTGTGCATTTCTCATTT38490.17638818047961635No Hit
CTGTAGGACCTGGAATATGGCGAGA29950.13725190972627982No Hit
CTGAAGGACCTGGAATATGGCGAGA29010.13294417032251676No Hit
ACGCAGAGTACTTTTTTTTTTTTTT25470.11672140703600492No Hit
ATTTAGAAATGTCCACTGTAGGACG24240.11108468419916603No Hit
GTCCTTCAGTGTGCATTTCTCATTT23390.10718938792980584No Hit
TTTCTAAATTTTCCACCTTTTTCAG22210.10178180016763522No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15000.014.021151
TCCAACG2000.013.76621318
TAGGACC11200.012.1395284
TAAAACG550.003050122212.1009424
GACGTTA550.003072208512.089297
GGCGAGG13650.012.03261219
CCAACGT1001.94544E-611.39272819
GTATTAG1950.011.2247291
CCAACGA1701.2732926E-1111.1693419
AGGACCT20250.011.0340915
GGACCTG20500.010.7500276
TGTAGGA35950.010.7114912
AGCGACT1355.738002E-810.54954615
TGGTATC4150.010.53912
TGGCGAG32300.010.49330118
TAGAAAT9150.010.3911284
GCCTTAA2200.010.3817651
CTGTAGG35300.010.3793141
CTAGTAC1659.1677066E-1010.3722363
TTTAGAA10250.010.2965772
Produced by FastQC (version 0.11.5)