FastQCFastQC Report
Thu 26 May 2016
SRR1512441_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512441_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2180864
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT68350.3134078970536448No Hit
GTATCAACGCAGAGTACTTTTTTTT66750.3060713552060101No Hit
GTCCTAAAGTGTGTATTTCTCATTT63610.291673391830027No Hit
CTGTAGGACGTGGAATATGGCAAGA60930.2793846842352389No Hit
CTTTAGGACGTGAAATATGGCGAGG56230.25783359255781196No Hit
GGTATCAACGCAGAGTACTTTTTTT45720.20964168329616154No Hit
TATCAACGCAGAGTACTTTTTTTTT41550.19052082110576357No Hit
GTCCTACAGTGTGCATTTCTCATTT38580.17690236530109169No Hit
CTGAAGGACCTGGAATATGGCGAGA28090.1288021628125367No Hit
CTGTAGGACCTGGAATATGGCGAGA27210.1247670647963376No Hit
ATTTAGAAATGTCCACTGTAGGACG25410.11651345521774856No Hit
GTCCTTCAGTGTGCATTTCTCATTT24920.11426663927691044No Hit
TTTCTAAATTTTCCACCTTTTTCAG22500.10317011973236295No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTTAA457.016944E-414.706348
GGTATCA12600.014.49049951
GACCGTC400.00533632214.2272227
CCGTGCA500.00155108113.240889
TAGGACC11450.012.92194754
GGCGAGG18450.012.32461319
CGACTAA1157.2022885E-811.55072717
GACGTTA752.1055847E-411.3817787
CCACCTT8800.011.3173713
GTGTAGG4900.011.2790921
AGGACCT21900.011.2599915
ATCCTAC3650.011.0859571
GGACCTG21600.011.0211996
TCCAACG1553.2923708E-1010.99940518
CACCTTT9450.010.944447514
CTAGGAC3300.010.9214053
ACCTTTT9500.010.88684615
GAACAGT4600.010.7214716
TGCGATA803.8461146E-410.66500710
AATGTCC10400.010.6358368
Produced by FastQC (version 0.11.5)