FastQCFastQC Report
Thu 26 May 2016
SRR1512441_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512441_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2180864
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT81910.37558508921234884No Hit
GTCCTACAGTGGACATTTCTAAATT72520.33252875924404274No Hit
CTGTAGGACGTGGAATATGGCAAGA70550.3234956420941425No Hit
GTCCTAAAGTGTGTATTTCTCATTT69300.3177639687756779No Hit
CTTTAGGACGTGAAATATGGCGAGG67810.3109318141800681No Hit
GGTATCAACGCAGAGTACTTTTTTT58470.26810475114450055No Hit
TATCAACGCAGAGTACTTTTTTTTT56700.25998870172555466No Hit
GTCCTACAGTGTGCATTTCTCATTT41300.18937448644207067No Hit
CTGAAGGACCTGGAATATGGCGAGA33850.1552137134640216No Hit
CTGTAGGACCTGGAATATGGCGAGA30930.14182452459208827No Hit
ATTTAGAAATGTCCACTGTAGGACG29000.1329748209883789No Hit
TTTCTAAATTTTCCACCTTTTTCAG27970.12825192217396408No Hit
ACGCAGAGTACTTTTTTTTTTTTTT26160.11995245920882734No Hit
GTCCTTCAGTGTGCATTTCTCATTT25880.11866856438549125No Hit
GAATATGGCAAGAAAACTGAAAATC24920.11426663927691044No Hit
GTACTTTTTTTTTTTTTTTTTTTTT22160.10161110458974057No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGAG456.7163416E-414.791535
TAGGACC11450.014.03391554
GGTATCA15150.013.8158311
AGCACCG500.001489409113.3123755
TCCAACG1850.012.31684418
ACCTTTT12150.011.79968815
CCACCTT12250.011.39621613
CACCTTT12450.011.28683614
TGGTATC4050.011.268962
TTCCACC11950.011.2075411
AGGACCT23800.010.9871065
TGTCCAC11900.010.93535110
GAAATGT11600.010.8911246
CGTACAG700.001480288110.8672475
AAATGTC11450.010.7846727
GGACCTG23200.010.7682916
ATAAGGG1158.7235094E-710.74838353
GCCGGTT1158.8376873E-710.73753511
TCCACCT13150.010.68871112
AATGTCC12000.010.6861588
Produced by FastQC (version 0.11.5)