##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512441_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2180864 Sequences flagged as poor quality 0 Sequence length 25 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.847953838478695 34.0 31.0 34.0 30.0 34.0 2 32.02020391918065 34.0 31.0 34.0 30.0 34.0 3 32.14962831244864 34.0 31.0 34.0 30.0 34.0 4 35.58881388293814 37.0 35.0 37.0 33.0 37.0 5 35.39002294503463 37.0 35.0 37.0 33.0 37.0 6 35.4153216339946 37.0 35.0 37.0 33.0 37.0 7 35.325972183501584 37.0 35.0 37.0 33.0 37.0 8 35.29524353650663 37.0 35.0 37.0 33.0 37.0 9 36.937286323218686 39.0 37.0 39.0 33.0 39.0 10 36.858982495011155 39.0 37.0 39.0 33.0 39.0 11 36.897613973177606 39.0 37.0 39.0 33.0 39.0 12 36.825955676282426 39.0 37.0 39.0 33.0 39.0 13 36.803371049272215 39.0 37.0 39.0 33.0 39.0 14 38.120129911814765 40.0 38.0 41.0 33.0 41.0 15 38.12551814326799 40.0 38.0 41.0 33.0 41.0 16 38.080801920706655 40.0 38.0 41.0 33.0 41.0 17 38.073165497710995 40.0 38.0 41.0 33.0 41.0 18 38.03125917067731 40.0 38.0 41.0 33.0 41.0 19 38.062892963522714 40.0 38.0 41.0 33.0 41.0 20 38.044449814385494 40.0 38.0 41.0 33.0 41.0 21 37.993680027732125 40.0 38.0 41.0 33.0 41.0 22 37.9386302859784 40.0 38.0 41.0 33.0 41.0 23 37.86889966545369 40.0 38.0 41.0 33.0 41.0 24 37.82387897640568 40.0 38.0 41.0 33.0 41.0 25 37.75660884860312 40.0 38.0 41.0 33.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.4887089018306412 1101 2 0.5147723964936901 1101 3 0.44753459076602553 1101 4 0.3560095176994551 1101 5 0.36081822525365226 1101 6 0.446616228416552 1101 7 0.4657983909108694 1101 8 0.5425353367018957 1101 9 0.6143928442833655 1101 10 0.5200505759921157 1101 11 0.6071444267593407 1101 12 0.5293648880206661 1101 13 0.6642012162160071 1101 14 0.7885339457138016 1101 15 0.836501088157803 1101 16 0.7596578684496222 1101 17 0.7935916430105436 1101 18 0.669307181679784 1101 19 0.745268685910716 1101 20 0.7364778996599881 1101 21 0.7252198922121238 1101 22 0.7418028351997776 1101 23 0.6535279470129325 1101 24 0.6740323493971374 1101 25 0.6007771332378695 1102 1 0.6010724548678894 1102 2 0.5672917698792403 1102 3 0.5689661381370783 1102 4 0.46973504057255155 1102 5 0.48993704868502874 1102 6 0.5780023629053446 1102 7 0.6851330296909666 1102 8 0.6572953567002244 1102 9 0.7387437317094196 1102 10 0.7488315109142079 1102 11 0.7329685247511719 1102 12 0.738349139333053 1102 13 0.7722876256818836 1102 14 0.938266051371663 1102 15 0.9261994466279333 1102 16 1.0820930821818067 1102 17 0.9892628370777103 1102 18 0.9342959326172036 1102 19 1.0349437129917973 1102 20 1.0017845407732295 1102 21 0.9198299247094184 1102 22 0.8823873698218918 1102 23 0.927850920098507 1102 24 0.8819137759449234 1102 25 0.997591148736575 1103 1 0.3853500924983777 1103 2 0.4545994278693968 1103 3 0.35083306220609245 1103 4 0.31478666975094427 1103 5 0.2768399468948388 1103 6 0.34472161860158224 1103 7 0.3916270312970269 1103 8 0.4114050068547499 1103 9 0.5102134396172389 1103 10 0.5169532308593716 1103 11 0.5616456335171804 1103 12 0.5106279612306395 1103 13 0.7162004117107799 1103 14 0.6696320953517727 1103 15 0.7346989964442088 1103 16 0.6641510301551676 1103 17 0.7917412809831958 1103 18 0.7648743578664252 1103 19 0.7610933037707355 1103 20 0.7906090340123626 1103 21 0.5962536814317545 1103 22 0.8380337282005783 1103 23 0.8026832165221833 1103 24 0.8638593807728441 1103 25 0.8928889594647345 1104 1 0.33442553459546787 1104 2 0.3817626953514619 1104 3 0.3091837849453398 1104 4 0.2139535014265732 1104 5 0.2004763382250161 1104 6 0.21423710151935182 1104 7 0.2138636389158748 1104 8 0.34067099622309627 1104 9 0.32601622090674454 1104 10 0.419103932274389 1104 11 0.30345408096056303 1104 12 0.37349256757003246 1104 13 0.2997738965477197 1104 14 0.2794328660705503 1104 15 0.39154255271733973 1104 16 0.5057218646943369 1104 17 0.5522366124988878 1104 18 0.49591178189884033 1104 19 0.586997679808384 1104 20 0.5029847061065524 1104 21 0.49546163841772284 1104 22 0.4664711187234829 1104 23 0.5038095930777118 1104 24 0.5526405484270285 1104 25 0.5241091626542342 1105 1 0.3574836053879551 1105 2 0.44772770205769064 1105 3 0.3488951866918413 1105 4 0.28329896738021176 1105 5 0.15741901576748063 1105 6 0.29431613720341687 1105 7 0.3087719391018098 1105 8 0.31998744980617744 1105 9 0.4102852127844301 1105 10 0.4202178000723862 1105 11 0.42577014886332165 1105 12 0.404906085953165 1105 13 0.36681751296274 1105 14 0.5074728329135567 1105 15 0.5526245299334178 1105 16 0.5005000697266055 1105 17 0.6501209832355386 1105 18 0.48500192172235046 1105 19 0.5196180322165844 1105 20 0.42463691455813546 1105 21 0.49215817132431283 1105 22 0.5628487458109035 1105 23 0.6685325076696671 1105 24 0.5187769526200015 1105 25 0.6112088754087424 1106 1 0.4168150596864777 1106 2 0.4022153108749862 1106 3 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0.6218356869219903 1107 16 0.6241320415444065 1107 17 0.6424567938525954 1107 18 0.5673900539385741 1107 19 0.6996441150129371 1107 20 0.536027392814546 1107 21 0.6503002605332711 1107 22 0.6408963693866028 1107 23 0.693011408908653 1107 24 0.6930495538719157 1107 25 0.6619041071080645 1108 1 0.16052505689567909 1108 2 0.14251477774438115 1108 3 0.17083119687576698 1108 4 0.12885986961750007 1108 5 0.21093190767985703 1108 6 0.16746985998968 1108 7 0.1754087015568473 1108 8 0.14224035199466556 1108 9 0.24095049619772624 1108 10 0.17274615767026802 1108 11 0.09452530894970579 1108 12 0.1775662367327584 1108 13 0.25877191305058744 1108 14 0.3009190656607359 1108 15 0.30838044107080265 1108 16 0.44805986572271195 1108 17 0.44756039416211024 1108 18 0.3061612923036563 1108 19 0.3741491811754827 1108 20 0.3672021875326337 1108 21 0.3691950921236824 1108 22 0.3331259365005792 1108 23 0.3754592585064671 1108 24 0.5944378842757203 1108 25 0.4343477195222789 1109 1 0.18363004270840833 1109 2 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-1.0383906984294384 2315 13 -0.887587634619841 2315 14 -0.9509938763336052 2315 15 -1.0152580926092085 2315 16 -1.2499011495558747 2315 17 -1.2311531943974785 2315 18 -1.0303866232098429 2315 19 -0.9513848694112426 2315 20 -0.974501890784552 2315 21 -0.9340366606345043 2315 22 -0.8437451285857875 2315 23 -0.9088758371924186 2315 24 -1.020656369194974 2315 25 -1.02324324586084 2316 1 -1.1578255920442793 2316 2 -1.1112527485740067 2316 3 -0.9320269824125695 2316 4 -0.6998770071232414 2316 5 -0.7353745535987031 2316 6 -0.7248763570122065 2316 7 -0.838531073950513 2316 8 -0.9336187574309278 2316 9 -1.1837470654522875 2316 10 -1.0633794691400524 2316 11 -1.072891034233571 2316 12 -1.1959607083687587 2316 13 -1.14534423832945 2316 14 -1.311927047194004 2316 15 -1.3043951078447762 2316 16 -1.3681338788102124 2316 17 -1.4973496851648207 2316 18 -1.643955229667732 2316 19 -1.5723393089313191 2316 20 -1.6499211976965569 2316 21 -1.6907923579490642 2316 22 -1.7824363653160233 2316 23 -1.8985997125615341 2316 24 -1.7792010484107266 2316 25 -1.640734794550653 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 16829.0 3 0.0 4 376.0 5 2884.0 6 3732.0 7 2821.0 8 2271.0 9 2042.0 10 1953.0 11 1777.0 12 1710.0 13 1735.0 14 1639.0 15 1691.0 16 1757.0 17 1900.0 18 2050.0 19 2243.0 20 2994.0 21 3878.0 22 4958.0 23 6284.0 24 7702.0 25 9075.0 26 10565.0 27 11804.0 28 13695.0 29 16873.0 30 21950.0 31 28272.0 32 37176.0 33 49782.0 34 67251.0 35 92839.0 36 140076.0 37 265667.0 38 1340613.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.04899087794078 20.4935833249125 13.657275140781024 24.80015065636569 2 13.925428640867965 21.05226231583002 43.062369023216995 21.959940020085018 3 18.13612317692583 26.02478926920215 30.867505203215423 24.9715823506566 4 11.933636702120829 18.09579227124911 39.7858810625611 30.184689964068955 5 12.025239460413573 38.986381800779036 36.74186936352765 12.246509375279743 6 29.927841283849048 38.820352684210675 18.840800405835445 12.411005626104833 7 26.496365463440675 32.25534815094758 23.677759314746876 17.570527070864863 8 23.331491167925225 39.259953999371675 21.35204239579344 16.056512436909657 9 25.941205530197426 15.034289664295677 21.553522929879918 37.47098187562698 10 14.66006601265579 29.475898408761083 35.77365805150748 20.09037752707565 11 32.728882365083365 22.25751702942593 26.940437145937018 18.07316345955368 12 23.220347768092836 24.99354396593599 33.349051043262996 18.437057222708177 13 28.895331344641722 22.9405392216217 26.427904425389208 21.73622500834737 14 19.446115665187634 24.041091241926935 28.730027128804043 27.782765964081392 15 21.396114333091358 33.95709872283016 23.7691345043131 20.877652439765377 16 20.127017025783996 28.735057157661604 30.5119657375012 20.625960079053197 17 20.10211614737373 30.210193273265013 29.409083614187132 20.278606965174127 18 19.909906853003456 27.604002835586428 32.99959557127621 19.48649474013391 19 22.703535251870473 27.09184391683476 29.00347247855551 21.20114835273926 20 22.926418153539146 28.007752890597487 29.86243066967954 19.203398286183823 21 21.428876026486787 27.291606388486016 28.489298492659547 22.790219092367646 22 22.243509466684213 29.735875635617603 28.62589206572225 19.394722831975926 23 20.348902257845598 29.444067423561567 29.75252654940298 20.454503769189852 24 22.362696619321518 27.346409496419767 30.532669620847518 19.758224263411197 25 21.309306770160816 28.55061113393591 29.976789015729544 20.16329308017373 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 280.0 1 280.0 2 530.0 3 780.0 4 780.0 5 780.0 6 2057.5 7 3335.0 8 3335.0 9 3335.0 10 4532.0 11 5729.0 12 5729.0 13 5729.0 14 9145.5 15 12562.0 16 12562.0 17 12562.0 18 22380.0 19 32198.0 20 32198.0 21 32198.0 22 51751.5 23 71305.0 24 71305.0 25 71305.0 26 103681.0 27 136057.0 28 136057.0 29 136057.0 30 174225.0 31 212393.0 32 212393.0 33 212393.0 34 233213.0 35 254033.0 36 254033.0 37 254033.0 38 262032.5 39 270032.0 40 270032.0 41 270032.0 42 274903.5 43 279775.0 44 279775.0 45 279775.0 46 279107.5 47 278440.0 48 278440.0 49 278440.0 50 257315.5 51 236191.0 52 236191.0 53 236191.0 54 199562.5 55 162934.0 56 162934.0 57 162934.0 58 135040.5 59 107147.0 60 107147.0 61 107147.0 62 85666.5 63 64186.0 64 64186.0 65 64186.0 66 48211.0 67 32236.0 68 32236.0 69 32236.0 70 23009.0 71 13782.0 72 13782.0 73 13782.0 74 9379.0 75 4976.0 76 4976.0 77 4976.0 78 3368.0 79 1760.0 80 1760.0 81 1760.0 82 1146.0 83 532.0 84 532.0 85 532.0 86 338.5 87 145.0 88 145.0 89 145.0 90 94.0 91 43.0 92 43.0 93 43.0 94 27.0 95 11.0 96 11.0 97 11.0 98 6.5 99 2.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.17075801150369763 2 0.00614435379739406 3 4.5853386547716866E-5 4 9.170677309543373E-4 5 0.11596321457917597 6 0.0307217689869703 7 0.024348148256837655 8 0.021046704425402043 9 0.03489442716281254 10 0.030996889306256603 11 0.03732465664984153 12 0.033610532339476466 13 0.02457741518957624 14 0.007153128301443832 15 0.008391169738232188 16 0.0024760828735767106 17 6.419474116680362E-4 18 4.585338654771687E-4 19 4.5853386547716866E-5 20 0.0 21 1.375601596431506E-4 22 0.0050897259067965725 23 0.0011004812771452047 24 0.0 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2180864.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.6983965161466 #Duplication Level Percentage of deduplicated Percentage of total 1 83.86902410047529 56.77798448976231 2 11.317123732688101 15.32302259755624 3 2.5842202475680938 5.24842701014758 4 0.8688202897994685 2.3527096192047123 5 0.4070088208787949 1.3776922270710972 6 0.21448023216513817 0.8711980681194423 7 0.13241706307367454 0.6275095989025649 8 0.09734206234106516 0.5271921227251912 9 0.06721127671588022 0.40950860953213 >10 0.37882974381178225 4.740090215443137 >50 0.032072936847338356 1.5054740370969413 >100 0.02548787329467295 3.6544022267413796 >500 0.002709827427629752 1.2199948244548668 >1k 0.00277757311332037 3.1759490845709566 >5k 4.742197998351851E-4 2.1888452686715114 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 8191 0.37558508921234884 No Hit GTCCTACAGTGGACATTTCTAAATT 7252 0.33252875924404274 No Hit CTGTAGGACGTGGAATATGGCAAGA 7055 0.3234956420941425 No Hit GTCCTAAAGTGTGTATTTCTCATTT 6930 0.3177639687756779 No Hit CTTTAGGACGTGAAATATGGCGAGG 6781 0.3109318141800681 No Hit GGTATCAACGCAGAGTACTTTTTTT 5847 0.26810475114450055 No Hit TATCAACGCAGAGTACTTTTTTTTT 5670 0.25998870172555466 No Hit GTCCTACAGTGTGCATTTCTCATTT 4130 0.18937448644207067 No Hit CTGAAGGACCTGGAATATGGCGAGA 3385 0.1552137134640216 No Hit CTGTAGGACCTGGAATATGGCGAGA 3093 0.14182452459208827 No Hit ATTTAGAAATGTCCACTGTAGGACG 2900 0.1329748209883789 No Hit TTTCTAAATTTTCCACCTTTTTCAG 2797 0.12825192217396408 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 2616 0.11995245920882734 No Hit GTCCTTCAGTGTGCATTTCTCATTT 2588 0.11866856438549125 No Hit GAATATGGCAAGAAAACTGAAAATC 2492 0.11426663927691044 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 2216 0.10161110458974057 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 4.5853386547716866E-5 0.0 5 0.0 0.0 0.0 4.5853386547716866E-5 0.0 6 0.0 0.0 0.0 4.5853386547716866E-5 0.0 7 0.0 0.0 0.0 4.5853386547716866E-5 0.0 8 0.0 0.0 0.0 4.5853386547716866E-5 0.0 9 0.0 0.0 0.0 4.5853386547716866E-5 0.0 10 0.0 0.0 0.0 4.5853386547716866E-5 0.0 11 0.0 0.0 0.0 4.5853386547716866E-5 0.0 12 0.0 0.0 0.0 4.5853386547716866E-5 0.0 13 0.0 0.0 0.0 4.5853386547716866E-5 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACGGAG 45 6.7163416E-4 14.79153 5 TAGGACC 1145 0.0 14.0339155 4 GGTATCA 1515 0.0 13.815831 1 AGCACCG 50 0.0014894091 13.312375 5 TCCAACG 185 0.0 12.316844 18 ACCTTTT 1215 0.0 11.799688 15 CCACCTT 1225 0.0 11.396216 13 CACCTTT 1245 0.0 11.286836 14 TGGTATC 405 0.0 11.26896 2 TTCCACC 1195 0.0 11.20754 11 AGGACCT 2380 0.0 10.987106 5 TGTCCAC 1190 0.0 10.935351 10 GAAATGT 1160 0.0 10.891124 6 CGTACAG 70 0.0014802881 10.867247 5 AAATGTC 1145 0.0 10.784672 7 GGACCTG 2320 0.0 10.768291 6 ATAAGGG 115 8.7235094E-7 10.7483835 3 GCCGGTT 115 8.8376873E-7 10.737535 11 TCCACCT 1315 0.0 10.688711 12 AATGTCC 1200 0.0 10.686158 8 >>END_MODULE