FastQCFastQC Report
Thu 26 May 2016
SRR1512440_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512440_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1870077
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT59240.31677840003379537No Hit
GTCCTACAGTGGACATTTCTAAATT56550.30239396559606907No Hit
GTCCTAAAGTGTGTATTTCTCATTT53500.28608447673544996No Hit
CTGTAGGACGTGGAATATGGCAAGA48770.260791400567998No Hit
CTTTAGGACGTGAAATATGGCGAGG42600.2277981067089751No Hit
GGTATCAACGCAGAGTACTTTTTTT37030.19801323688810676No Hit
TATCAACGCAGAGTACTTTTTTTTT35580.19025954546256652No Hit
GTCCTACAGTGTGCATTTCTCATTT34420.18405659232213434No Hit
CTGTAGGACCTGGAATATGGCGAGA22560.12063674383461216No Hit
CTGAAGGACCTGGAATATGGCGAGA21530.11512894923578013No Hit
GTCCTTCAGTGTGCATTTCTCATTT20330.10871210115947097No Hit
ATTTAGAAATGTCCACTGTAGGACG19280.10309735909270046No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACTCG604.142193E-412.64862613
GGTATCA12000.012.4174331
TAGGACC10450.011.7975434
GTGTAGG3850.011.7905281
CGATACT658.1432436E-411.6718884
CCAACGT752.1234911E-411.37122419
GGCGAGG14750.011.37122419
TGTCGAG1501.8553692E-1011.36755918
TGTAGGA33450.011.1986452
TAGAACG855.4195534E-511.1569534
ATCTGCG855.4195534E-511.1569537
GTCCTAG2400.011.1016781
GCACCGT600.00595333811.0639776
GGACGTG32900.010.8093596
CTGTAGG30850.010.7798041
TTAGGAC19300.010.7118313
AGGACGT33650.010.6529835
TAGAAAT7400.010.6367974
GTAGGAC33450.010.6032733
GACGTGA17950.010.4608097
Produced by FastQC (version 0.11.5)