FastQCFastQC Report
Thu 26 May 2016
SRR1512440_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512440_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1870077
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT75440.40340584906396904No Hit
GTCCTACAGTGGACATTTCTAAATT58080.3105754468933632No Hit
CTGTAGGACGTGGAATATGGCAAGA57320.30651144311170075No Hit
GTCCTAAAGTGTGTATTTCTCATTT54610.2920200612060359No Hit
GGTATCAACGCAGAGTACTTTTTTT52940.2830899476331723No Hit
CTTTAGGACGTGAAATATGGCGAGG50670.27095141002215417No Hit
TATCAACGCAGAGTACTTTTTTTTT49790.2662457214328608No Hit
GTCCTACAGTGTGCATTTCTCATTT36010.19255891602324396No Hit
CTGAAGGACCTGGAATATGGCGAGA27900.1491917177741879No Hit
CTGTAGGACCTGGAATATGGCGAGA26290.1405824466051398No Hit
ACGCAGAGTACTTTTTTTTTTTTTT23890.12774875045252146No Hit
ATTTAGAAATGTCCACTGTAGGACG23400.12512853748802857No Hit
TTTCTAAATTTTCCACCTTTTTCAG22660.12117148117430458No Hit
GTCCTTCAGTGTGCATTTCTCATTT21190.11331084228082587No Hit
GAATATGGCAAGAAAACTGAAAATC20420.10919336476519416No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAACGT551.1340315E-515.53751319
TCCAACG1250.014.43262418
GGTATCA12700.014.0893381
GTCCCGC1001.0117219E-813.2960512
TAGGACC10750.013.089194
GGCGAGG15550.012.33453219
TCCCGCC1251.4188117E-912.15541413
TTCTACG657.965699E-411.7026072
TAGAAAT9350.011.5918484
GGACCTG19750.011.5898926
AGGACCT20650.011.4180275
AATCCCG2600.011.32113619
ATTTAGA9450.011.2803541
ATCGCGC600.005885907411.080938
TGGTATC3850.010.8667062
ACTGTTC5900.010.7857978
AATGTCC8600.010.7127928
CGCCCTA1252.2562381E-710.63456516
TTTAGAA10650.010.6243732
TGTCCAC9700.010.57474310
Produced by FastQC (version 0.11.5)