##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512440_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1870077 Sequences flagged as poor quality 0 Sequence length 25 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.78682802900629 33.0 31.0 34.0 30.0 34.0 2 31.96215075635923 34.0 31.0 34.0 30.0 34.0 3 32.08848619602294 34.0 31.0 34.0 30.0 34.0 4 35.533095696059576 37.0 35.0 37.0 33.0 37.0 5 35.33376058846775 37.0 35.0 37.0 33.0 37.0 6 35.36070386406549 37.0 35.0 37.0 33.0 37.0 7 35.268355794975285 37.0 35.0 37.0 33.0 37.0 8 35.23428874853816 37.0 35.0 37.0 33.0 37.0 9 36.863030238861825 39.0 37.0 39.0 33.0 39.0 10 36.78687294694283 39.0 37.0 39.0 33.0 39.0 11 36.82703118641639 39.0 37.0 39.0 33.0 39.0 12 36.75151504456768 39.0 37.0 39.0 33.0 39.0 13 36.73098433914753 39.0 37.0 39.0 33.0 39.0 14 38.032999175969756 40.0 38.0 41.0 33.0 41.0 15 38.03105326679062 40.0 38.0 41.0 33.0 41.0 16 37.987319238726535 40.0 38.0 41.0 33.0 41.0 17 37.98252638795087 40.0 38.0 41.0 33.0 41.0 18 37.936653945265356 40.0 38.0 41.0 33.0 41.0 19 37.96548751735891 40.0 38.0 41.0 33.0 41.0 20 37.94394669310408 40.0 38.0 41.0 33.0 41.0 21 37.89316268795349 40.0 38.0 41.0 33.0 41.0 22 37.83500839804992 40.0 38.0 41.0 33.0 41.0 23 37.76236272624068 40.0 38.0 41.0 33.0 41.0 24 37.71796776282474 40.0 38.0 41.0 33.0 41.0 25 37.65247259872187 40.0 38.0 41.0 33.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3852252777059668 1101 2 0.3895008438487082 1101 3 0.33370050453825684 1101 4 0.34848761926414085 1101 5 0.23007819657069462 1101 6 0.35980419079213277 1101 7 0.33929005004110024 1101 8 0.3443398421419488 1101 9 0.4053718701169444 1101 10 0.40253718965681173 1101 11 0.4165664659193524 1101 12 0.4652546181556403 1101 13 0.4339485493073809 1101 14 0.6129734234640623 1101 15 0.5498134511368917 1101 16 0.6848827382745881 1101 17 0.5774914974130425 1101 18 0.47516608433394936 1101 19 0.5510251257814573 1101 20 0.5247672473372447 1101 21 0.619528930313642 1101 22 0.5501222268458577 1101 23 0.6098580454392248 1101 24 0.6276004684573238 1101 25 0.6038233281120142 1102 1 0.5350032610520365 1102 2 0.582619764812641 1102 3 0.5557087383030819 1102 4 0.4841194267175055 1102 5 0.4035005978812194 1102 6 0.49960253062496207 1102 7 0.4988589489528934 1102 8 0.571591228639079 1102 9 0.6888122121965168 1102 10 0.6823072086834898 1102 11 0.6387730092043995 1102 12 0.59258036346408 1102 13 0.6062037408502761 1102 14 0.7622069992692531 1102 15 0.7887842624725252 1102 16 0.8079737187917715 1102 17 0.8519710365452013 1102 18 0.8081202196681474 1102 19 0.8244233989497758 1102 20 0.8712714686007246 1102 21 0.8223362730502757 1102 22 0.9369944850280163 1102 23 0.8986832313870678 1102 24 0.8335981845588023 1102 25 0.9141356264090206 1103 1 0.3341557769228167 1103 2 0.4187832573228789 1103 3 0.3837101142920787 1103 4 0.22971782556667364 1103 5 0.2353641247911824 1103 6 0.21202083127855786 1103 7 0.3422307474330495 1103 8 0.3079642467458399 1103 9 0.40482684771579613 1103 10 0.4018712703528138 1103 11 0.3978342094420668 1103 12 0.48020271703070705 1103 13 0.5626099077827647 1103 14 0.6461704730160136 1103 15 0.6847250949458683 1103 16 0.7415509774464226 1103 17 0.7566212538889232 1103 18 0.7038108723247447 1103 19 0.7023981933332379 1103 20 0.7011022844996546 1103 21 0.6349203178324601 1103 22 0.6157323278525411 1103 23 0.6533975573088213 1103 24 0.5759755182674056 1103 25 0.5656976866398153 1104 1 0.2863521673878573 1104 2 0.3105562979250962 1104 3 0.3056301683640896 1104 4 0.2436131719629202 1104 5 0.16963827591188618 1104 6 0.27405540481613855 1104 7 0.15389432745439535 1104 8 0.3011814019628929 1104 9 0.3029530388991333 1104 10 0.3915837484340017 1104 11 0.3174733596116397 1104 12 0.28072379252056834 1104 13 0.24943747393571414 1104 14 0.27639217760098944 1104 15 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0.7621405452554342 1107 15 0.8150188303737664 1107 16 0.7735283882938049 1107 17 0.8029796607994442 1107 18 0.9011681453184082 1107 19 0.8379554051718401 1107 20 0.9560790965370103 1107 21 0.8989971311438794 1107 22 0.9325076084651158 1107 23 0.9507290305088887 1107 24 0.9019670584832369 1107 25 0.9655291916914805 1108 1 0.28788045997451306 1108 2 0.25161789073519714 1108 3 0.30512884350090275 1108 4 0.16279979107854814 1108 5 0.34884718340716603 1108 6 0.22238831258452052 1108 7 0.21998152430951734 1108 8 0.26986847329973784 1108 9 0.3449338519994214 1108 10 0.2745802775075745 1108 11 0.22912373789679208 1108 12 0.30280967807446046 1108 13 0.3403713504956869 1108 14 0.43509212812321607 1108 15 0.4869531017028166 1108 16 0.5756957791483472 1108 17 0.5616612940155292 1108 18 0.4459844544891567 1108 19 0.547981383974971 1108 20 0.48863593953768003 1108 21 0.5570741249595486 1108 22 0.35567184967715093 1108 23 0.3998749486181836 1108 24 0.55058650350243 1108 25 0.49518353566922 1109 1 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-0.6446767377122598 2315 11 -0.808470755706054 2315 12 -0.8300348591928568 2315 13 -0.7151022060553345 2315 14 -0.9019391553170024 2315 15 -0.8240147981468766 2315 16 -1.0763236103362672 2315 17 -0.9337348718750178 2315 18 -0.915180365969924 2315 19 -0.8887340392997913 2315 20 -0.7802018967709898 2315 21 -0.8325937544723985 2315 22 -0.842207533067139 2315 23 -0.8322090888815978 2315 24 -0.7582916517167604 2315 25 -0.7922412199942457 2316 1 -1.4794223411388963 2316 2 -1.413536264030995 2316 3 -1.1350993664164868 2316 4 -0.9588488817917167 2316 5 -0.9909584724241398 2316 6 -1.1483585765407511 2316 7 -1.1992871932869917 2316 8 -1.201755351659294 2316 9 -1.452591966097117 2316 10 -1.4008655191471746 2316 11 -1.5310848449626135 2316 12 -1.576999718903025 2316 13 -1.5782999004710092 2316 14 -1.8212552568435143 2316 15 -1.7927582995797806 2316 16 -1.9399967935597147 2316 17 -2.0299765462821995 2316 18 -2.217000393868787 2316 19 -2.28061339684691 2316 20 -2.1715430491802508 2316 21 -2.2291047129957775 2316 22 -2.1829847996518126 2316 23 -2.3943885341655644 2316 24 -2.2229303693460594 2316 25 -2.070054096911136 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 15469.0 3 2.0 4 311.0 5 2555.0 6 3195.0 7 2637.0 8 1918.0 9 1840.0 10 1703.0 11 1568.0 12 1437.0 13 1563.0 14 1509.0 15 1521.0 16 1560.0 17 1584.0 18 1814.0 19 2125.0 20 2708.0 21 3549.0 22 4452.0 23 5626.0 24 6870.0 25 8233.0 26 9413.0 27 10477.0 28 12306.0 29 15039.0 30 18861.0 31 24872.0 32 32885.0 33 43542.0 34 59315.0 35 81453.0 36 122535.0 37 233040.0 38 1130590.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.436580384645666 20.2795689594685 13.452475258661275 24.831375397224555 2 14.156155847822808 21.143350349576306 42.61381684704312 22.086676955557767 3 18.384751002231457 25.877116289864 30.689966242031748 25.048166465872796 4 12.026685803328025 17.993399135213988 39.55032410759453 30.42959095386346 5 12.08799797853478 38.842325712866284 36.735151652719125 12.334524655879807 6 30.15586295357061 38.71556816242825 18.686223841645507 12.442345042355633 7 26.715567634006533 32.11359045029789 23.474924704312993 17.69591721138258 8 23.541568500136652 39.071197097711554 21.173002898330584 16.214231503821207 9 26.043263598773986 15.007087570273928 21.275976613692652 37.67367221725944 10 14.57097956821418 29.461342855828555 35.723264250312106 20.24441332564516 11 33.06771224768716 22.2068998718878 26.605508022391554 18.119879858033492 12 23.250030757051388 25.012757490010646 33.18621656173609 18.550995191201878 13 29.0424324500683 22.77752996008887 26.304385731263647 21.875651858579182 14 19.57938779886638 23.76454658195098 28.665770738221152 27.990294880961486 15 21.578915281212296 33.64547056961213 23.779776929724736 20.995837219450838 16 20.29553425948257 28.716490636281527 30.238011333465593 20.74996377077031 17 20.221392900475866 29.9976631878289 29.275711217072736 20.505232694622496 18 20.013421993353173 27.572945325429863 32.67340974778952 19.740222933427447 19 22.754089804858303 27.001936283907025 28.930145657103957 21.313828254130712 20 22.871423407390928 27.98052057777331 29.708739110068255 19.439316904767505 21 21.5503541032557 27.171409076422563 28.519803473227828 22.758433347093916 22 22.2699469834469 29.528305620054095 28.632414643563415 19.56933275293559 23 20.59177875326795 29.12216044751577 29.66727752148731 20.618783277728966 24 22.310964901961203 27.28086986839038 30.478440448409582 19.929724781238836 25 21.37623210167282 28.4584003760273 29.864759579418386 20.300607942881495 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 241.0 1 241.0 2 476.0 3 711.0 4 711.0 5 711.0 6 1789.0 7 2867.0 8 2867.0 9 2867.0 10 4146.5 11 5426.0 12 5426.0 13 5426.0 14 8009.0 15 10592.0 16 10592.0 17 10592.0 18 18895.0 19 27198.0 20 27198.0 21 27198.0 22 43572.0 23 59946.0 24 59946.0 25 59946.0 26 86765.0 27 113584.0 28 113584.0 29 113584.0 30 145740.0 31 177896.0 32 177896.0 33 177896.0 34 195388.0 35 212880.0 36 212880.0 37 212880.0 38 220541.5 39 228203.0 40 228203.0 41 228203.0 42 232960.0 43 237717.0 44 237717.0 45 237717.0 46 237781.5 47 237846.0 48 237846.0 49 237846.0 50 221623.5 51 205401.0 52 205401.0 53 205401.0 54 175075.0 55 144749.0 56 144749.0 57 144749.0 58 120683.5 59 96618.0 60 96618.0 61 96618.0 62 77422.5 63 58227.0 64 58227.0 65 58227.0 66 43858.0 67 29489.0 68 29489.0 69 29489.0 70 21354.5 71 13220.0 72 13220.0 73 13220.0 74 9027.5 75 4835.0 76 4835.0 77 4835.0 78 3272.0 79 1709.0 80 1709.0 81 1709.0 82 1105.5 83 502.0 84 502.0 85 502.0 86 331.5 87 161.0 88 161.0 89 161.0 90 101.5 91 42.0 92 42.0 93 42.0 94 27.5 95 13.0 96 13.0 97 13.0 98 8.5 99 4.0 100 4.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1636296259458835 2 0.005293899662955055 3 0.0 4 0.0010160009454156166 5 0.11363168468464134 6 0.029410553683083637 7 0.023956232818220853 8 0.020320018908312334 9 0.03245855651933049 10 0.028822342609421965 11 0.03326066252886913 12 0.031389081839945626 13 0.022619389468989778 14 0.007272427819817045 15 0.009250955976679034 16 0.002138949358769719 17 4.2778987175394386E-4 18 6.416848076309157E-4 19 0.0 20 5.347373396924298E-5 21 5.347373396924298E-5 22 0.0050800047270780834 23 0.0010694746793848595 24 5.347373396924298E-5 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1870077.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.97044188224801 #Duplication Level Percentage of deduplicated Percentage of total 1 86.19340743616561 61.17184213081316 2 9.976146718649893 14.16023081809443 3 2.0262546168748057 4.314125565766504 4 0.6553136787470387 1.8603160540863541 5 0.30487329887339 1.0818496369571577 6 0.18521460816084317 0.7886857550533475 7 0.12292046247587482 0.6106603676798185 8 0.0784964582758522 0.44567426640229396 9 0.06837799387620219 0.4367534796374146 >10 0.33323916212549953 4.368755126482954 >50 0.026163212690556947 1.2894804060290617 >100 0.024309070842063317 3.535202012213746 >500 0.0027925920424092857 1.442030577256946 >1k 0.0020378374363527207 2.624644562052415 >5k 4.52852763633938E-4 1.8697492414743486 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 7544 0.40340584906396904 No Hit GTCCTACAGTGGACATTTCTAAATT 5808 0.3105754468933632 No Hit CTGTAGGACGTGGAATATGGCAAGA 5732 0.30651144311170075 No Hit GTCCTAAAGTGTGTATTTCTCATTT 5461 0.2920200612060359 No Hit GGTATCAACGCAGAGTACTTTTTTT 5294 0.2830899476331723 No Hit CTTTAGGACGTGAAATATGGCGAGG 5067 0.27095141002215417 No Hit TATCAACGCAGAGTACTTTTTTTTT 4979 0.2662457214328608 No Hit GTCCTACAGTGTGCATTTCTCATTT 3601 0.19255891602324396 No Hit CTGAAGGACCTGGAATATGGCGAGA 2790 0.1491917177741879 No Hit CTGTAGGACCTGGAATATGGCGAGA 2629 0.1405824466051398 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 2389 0.12774875045252146 No Hit ATTTAGAAATGTCCACTGTAGGACG 2340 0.12512853748802857 No Hit TTTCTAAATTTTCCACCTTTTTCAG 2266 0.12117148117430458 No Hit GTCCTTCAGTGTGCATTTCTCATTT 2119 0.11331084228082587 No Hit GAATATGGCAAGAAAACTGAAAATC 2042 0.10919336476519416 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 5.3473733969242976E-5 0.0 4 0.0 0.0 0.0 5.3473733969242976E-5 0.0 5 0.0 0.0 0.0 5.3473733969242976E-5 0.0 6 0.0 0.0 0.0 5.3473733969242976E-5 0.0 7 0.0 0.0 0.0 5.3473733969242976E-5 0.0 8 0.0 0.0 0.0 5.3473733969242976E-5 0.0 9 0.0 0.0 0.0 1.6042120190772893E-4 0.0 10 0.0 0.0 0.0 1.6042120190772893E-4 0.0 11 0.0 0.0 0.0 1.6042120190772893E-4 0.0 12 0.0 5.3473733969242976E-5 0.0 1.6042120190772893E-4 0.0 13 0.0 5.3473733969242976E-5 0.0 1.6042120190772893E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCAACGT 55 1.1340315E-5 15.537513 19 TCCAACG 125 0.0 14.432624 18 GGTATCA 1270 0.0 14.089338 1 GTCCCGC 100 1.0117219E-8 13.29605 12 TAGGACC 1075 0.0 13.08919 4 GGCGAGG 1555 0.0 12.334532 19 TCCCGCC 125 1.4188117E-9 12.155414 13 TTCTACG 65 7.965699E-4 11.702607 2 TAGAAAT 935 0.0 11.591848 4 GGACCTG 1975 0.0 11.589892 6 AGGACCT 2065 0.0 11.418027 5 AATCCCG 260 0.0 11.321136 19 ATTTAGA 945 0.0 11.280354 1 ATCGCGC 60 0.0058859074 11.08093 8 TGGTATC 385 0.0 10.866706 2 ACTGTTC 590 0.0 10.785797 8 AATGTCC 860 0.0 10.712792 8 CGCCCTA 125 2.2562381E-7 10.634565 16 TTTAGAA 1065 0.0 10.624373 2 TGTCCAC 970 0.0 10.574743 10 >>END_MODULE