FastQCFastQC Report
Thu 26 May 2016
SRR1512439_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512439_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1796599
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT76080.4234667836284001No Hit
GTCCTAAAGTGTGTATTTCTCATTT72340.4026496730767411No Hit
CTGTAGGACGTGGAATATGGCAAGA71910.40025626197053427No Hit
CTTTAGGACGTGAAATATGGCGAGG56930.316876498317098No Hit
GTCCTACAGTGTGCATTTCTCATTT45480.25314497002391745No Hit
GTATCAACGCAGAGTACTTTTTTTT40790.22704009074924342No Hit
CTGAAGGACCTGGAATATGGCGAGA31720.17655581462530037No Hit
ATTTAGAAATGTCCACTGTAGGACG30750.17115672445548505No Hit
GTCCTTCAGTGTGCATTTCTCATTT30350.16893029551947875No Hit
CTGTAGGACCTGGAATATGGCGAGA30250.16837368828547716No Hit
GGTATCAACGCAGAGTACTTTTTTT27870.15512643611623964No Hit
TATCAACGCAGAGTACTTTTTTTTT24570.13675839739418758No Hit
GAATATGGCAAGAAAACTGAAAATC24280.135144236415583No Hit
TTTCTAAATTTTCCACCTTTTTCAG23810.1325281824157756No Hit
GGAATATGGCGAGAAAACTGAAAAT19970.11115446463011501No Hit
ATTCCAGGTCCTTCAGTGTGCATTT18020.10030062356708425No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA452.5411444E-517.553471
TAGGACC12550.014.281654
GACCGTG602.6116055E-514.2249777
ATAGGAC3300.013.5065443
CGTGCGC852.7732312E-713.3822210
ACCTTTT9550.013.1159115
GGTATCA9750.013.0638321
TCCCCCG957.5084245E-812.98407717
GTCGAGG1251.07320375E-1012.88757219
TGTCGAG1750.012.45019818
CCACCTT9900.012.36154813
TATGTCG1700.012.27940316
CACCTTT10250.012.03503314
TCCAACG1750.011.90888518
ATGTCGA2000.011.860455517
AGGACCT24400.011.5820855
AAATGTC10200.011.5287397
AATGTCC10500.011.5232028
GGCGAGG18700.011.401793519
CCCGTTT1501.8189894E-1011.37743412
Produced by FastQC (version 0.11.5)