FastQCFastQC Report
Thu 26 May 2016
SRR1512439_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512439_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1796599
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGA83730.4660472370295207No Hit
GTCCTACAGTGGACATTTCTAAATT82340.45831039647689886No Hit
GTCCTAAAGTGTGTATTTCTCATTT78140.43493289264883256No Hit
CTTTAGGACGTGAAATATGGCGAGG67500.3757098829510648No Hit
GTATCAACGCAGAGTACTTTTTTTT50670.2820328854685993No Hit
GTCCTACAGTGTGCATTTCTCATTT46980.2614940785339411No Hit
CTGAAGGACCTGGAATATGGCGAGA39290.21869098223921976No Hit
CTGTAGGACCTGGAATATGGCGAGA36350.20232672955957343No Hit
GGTATCAACGCAGAGTACTTTTTTT34840.19392196032614958No Hit
ATTTAGAAATGTCCACTGTAGGACG33790.188077584369133No Hit
TATCAACGCAGAGTACTTTTTTTTT33460.1862407804969278No Hit
GTCCTTCAGTGTGCATTTCTCATTT31920.1776690290933035No Hit
TTTCTAAATTTTCCACCTTTTTCAG29940.16664820586007228No Hit
GAATATGGCAAGAAAACTGAAAATC26790.1491150779890226No Hit
GGAATATGGCGAGAAAACTGAAAAT23640.1315819501179729No Hit
ATTCCAGGTCCTTCAGTGTGCATTT20430.11371485790652226No Hit
GCCATATTCCACGTCCTACAGTGGA19540.10876105352390823No Hit
TTCCAGGTCCTTCAGTGTGCATTTC18560.10330630263069276No Hit
ACAGTGGACATTTCTAAATTTTCCA18120.10085723080108583No Hit
GACCTGGAATATGGCGAGAAAACTG17990.10013364139688377No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTAGG555.8046135E-717.302681
AATACCG456.724366E-414.7889625
GGTATCA11600.013.9465561
TAGGACC13500.013.380494
TAGAAAT11050.012.9056884
CGTGCGC604.100121E-412.66390110
AGGCGTG1207.4396667E-1012.6639017
AAATGTC10550.012.4238267
AAGGCGT1155.340553E-912.3889456
GCCGGTT1155.342372E-912.38859911
CACCTTT9250.012.3188914
TGTCGAG2100.012.20787918
ACCTTTT9200.012.17940915
TCCAACG1251.4224497E-912.15362318
GTCCTAG2600.012.0786011
GAAATGT11350.011.6321456
AATGTCC11200.011.6179988
GTCGAGG1800.011.60502119
CCCCGGT752.0842874E-411.39402219
AGGACCT25800.011.38651855
Produced by FastQC (version 0.11.5)