##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512439_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1796599 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.926006304133534 34.0 31.0 34.0 30.0 34.0 2 32.099155682486746 34.0 31.0 34.0 30.0 34.0 3 32.22532796689745 34.0 31.0 34.0 30.0 34.0 4 35.655175139249216 37.0 35.0 37.0 35.0 37.0 5 35.4692226812995 37.0 35.0 37.0 33.0 37.0 6 35.497442111456145 37.0 35.0 37.0 33.0 37.0 7 35.41292853886705 37.0 35.0 37.0 33.0 37.0 8 35.378147266028755 37.0 35.0 37.0 33.0 37.0 9 37.033756558920494 39.0 37.0 39.0 34.0 39.0 10 36.95370141027575 39.0 37.0 39.0 33.0 39.0 11 36.993907377216615 39.0 37.0 39.0 33.0 39.0 12 36.91886002385618 39.0 37.0 39.0 33.0 39.0 13 36.90325442683648 39.0 37.0 39.0 33.0 39.0 14 38.2298615328184 40.0 38.0 41.0 33.0 41.0 15 38.24664936360312 41.0 38.0 41.0 33.0 41.0 16 38.20094189076138 41.0 38.0 41.0 33.0 41.0 17 38.19099309306083 40.0 38.0 41.0 33.0 41.0 18 38.14668882705601 40.0 38.0 41.0 33.0 41.0 19 38.167049519675786 40.0 38.0 41.0 33.0 41.0 20 38.15433549723672 40.0 39.0 41.0 34.0 41.0 21 38.10349833212642 40.0 38.0 41.0 33.0 41.0 22 38.05065626775925 40.0 38.0 41.0 33.0 41.0 23 37.97854780059434 40.0 38.0 41.0 33.0 41.0 24 37.93652562424893 40.0 38.0 41.0 33.0 41.0 25 37.87485910879389 40.0 38.0 41.0 33.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.4047425516557297 1101 2 0.4374203516855246 1101 3 0.42997709303308085 1101 4 0.38871192022737233 1101 5 0.254711260818695 1101 6 0.37407821927055807 1101 7 0.4645681111106512 1101 8 0.4115854123369118 1101 9 0.49762084960138964 1101 10 0.4911662795772429 1101 11 0.43153822731347447 1101 12 0.45170520720837004 1101 13 0.5672898053463413 1101 14 0.6422893777502097 1101 15 0.6830706748277606 1101 16 0.675431176783114 1101 17 0.6659000207421499 1101 18 0.5097926742611278 1101 19 0.5933726825117915 1101 20 0.5701251620413501 1101 21 0.6006131412977069 1101 22 0.5686581464803382 1101 23 0.5222016081795076 1101 24 0.5560404195983324 1101 25 0.6206882349369991 1102 1 0.5027576148156143 1102 2 0.5113153658949159 1102 3 0.5187166610687441 1102 4 0.3945097598815295 1102 5 0.3615932816883287 1102 6 0.4623593004116415 1102 7 0.4405775608122937 1102 8 0.498605114940176 1102 9 0.6541230540076057 1102 10 0.576316268307778 1102 11 0.6197028521666752 1102 12 0.6580665509973826 1102 13 0.6734028508863901 1102 14 0.7888220359227915 1102 15 0.8096463573858657 1102 16 0.878649711616859 1102 17 0.7887378172054156 1102 18 0.7775184960148707 1102 19 0.9235214795463946 1102 20 0.9413219485014608 1102 21 0.8021694684322682 1102 22 0.826560262149151 1102 23 0.7849392651850593 1102 24 0.8114700360017437 1102 25 0.8049777667901168 1103 1 0.49197510193561556 1103 2 0.4770867353013628 1103 3 0.43796833258977586 1103 4 0.3834277327548534 1103 5 0.3686251875250903 1103 6 0.383681152662362 1103 7 0.42426516596881214 1103 8 0.3926708739015581 1103 9 0.5310793955761497 1103 10 0.5641243464652632 1103 11 0.5718479559844241 1103 12 0.5234537466297624 1103 13 0.5653752753903944 1103 14 0.6729088350921089 1103 15 0.7377659066124735 1103 16 0.7315891070314748 1103 17 0.6964149996223554 1103 18 0.7830038515789468 1103 19 0.8921683638264355 1103 20 0.7686384789569303 1103 21 0.7606883779035698 1103 22 0.7728450981198307 1103 23 0.8102305568899695 1103 24 0.8367013654887856 1103 25 0.8134582648208664 1104 1 0.4065955060042441 1104 2 0.4400530277964805 1104 3 0.36132767699174906 1104 4 0.30326161158007636 1104 5 0.23552154101299294 1104 6 0.19863457780934368 1104 7 0.3280240211458647 1104 8 0.31765867763441236 1104 9 0.4015000694514015 1104 10 0.49376505474200627 1104 11 0.34405088812737716 1104 12 0.3557384681998599 1104 13 0.2700561225557081 1104 14 0.2504536177237071 1104 15 0.316375142794044 1104 16 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-0.8404781056976134 2315 13 -0.8453003038667575 2315 14 -0.9915904311080439 2315 15 -0.9091062111203456 2315 16 -1.011326679043151 2315 17 -1.0500141311680409 2315 18 -0.9104181500414086 2315 19 -0.7490644199143404 2315 20 -0.7309748017568012 2315 21 -0.8301592334280841 2315 22 -0.769115394637879 2315 23 -0.6667117890652392 2315 24 -0.9207632835344555 2315 25 -0.8798660901797035 2316 1 -1.0618688000246976 2316 2 -0.9468068002700853 2316 3 -0.7946018950611666 2316 4 -0.7109686508444284 2316 5 -0.5868091439971579 2316 6 -0.6553275556744751 2316 7 -0.7315769296208003 2316 8 -0.7606099094578767 2316 9 -1.0387299291888468 2316 10 -1.0156932034407191 2316 11 -0.9841875842759364 2316 12 -1.019669611356818 2316 13 -1.0522500623684934 2316 14 -1.3085307475464418 2316 15 -1.223484863714809 2316 16 -1.332796531489798 2316 17 -1.4344321067331194 2316 18 -1.662644308659111 2316 19 -1.5779350367578857 2316 20 -1.5137487413862019 2316 21 -1.764128219748983 2316 22 -1.5939344389935144 2316 23 -1.7890900996908528 2316 24 -1.6443623057517414 2316 25 -1.4996723951614541 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 12317.0 3 0.0 4 268.0 5 2113.0 6 2854.0 7 2150.0 8 1685.0 9 1584.0 10 1471.0 11 1404.0 12 1236.0 13 1265.0 14 1223.0 15 1349.0 16 1342.0 17 1420.0 18 1572.0 19 1822.0 20 2306.0 21 2950.0 22 3913.0 23 5007.0 24 6124.0 25 7205.0 26 8322.0 27 9459.0 28 11053.0 29 13460.0 30 17483.0 31 22858.0 32 30275.0 33 40428.0 34 54875.0 35 75849.0 36 114337.0 37 214111.0 38 1119509.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.061610581694346 19.804938480855135 12.74194519588668 25.391505741563837 2 14.132505212861771 20.52475956266511 43.2678102863428 22.074924938130316 3 18.64945933956325 25.418415572979836 30.267243831261176 25.66488125619573 4 11.977869062329537 17.49479566732347 39.269500940676174 31.25783432967082 5 12.045909345982404 38.862453071703264 36.76759953925705 12.324038043057287 6 30.688749531603992 38.27267245464006 18.611454307656416 12.427123706099533 7 27.465439583994478 31.55050024218737 23.03652853635316 17.947531637464994 8 23.673298734788904 39.79252007825313 20.72344064375116 15.810740543206798 9 26.423644925224295 14.653298967522305 20.855960369070548 38.06709573818286 10 14.876372188530743 28.896135712201758 35.79789046544724 20.42960163382026 11 33.72699816920795 21.302947085209784 27.107582419050928 17.86247232653134 12 24.326391982182628 24.05501113585746 33.1989420935412 18.419654788418708 13 29.91141071422606 22.408100864402495 25.97032325735989 21.71016516401155 14 19.415775519394458 23.985467030566106 27.98247894339278 28.61627850664666 15 21.441080267461718 34.799080850836425 22.932025713019723 20.82781316868213 16 20.246415371599056 28.048937970343324 30.932504341630672 20.77214231642695 17 20.349807718746714 30.103969359680327 29.225080666842185 20.321142254730777 18 20.210765716423094 26.843379019833108 33.4446552138716 19.501200049872203 19 23.478318466345836 26.357537968983603 28.144860452922693 22.01928311174787 20 23.65235648021623 27.61000089613765 29.310213353118865 19.42742927052726 21 21.58574149587164 26.85726022181924 27.711263176292082 23.845735106017038 22 22.904138869638548 30.065321276589824 27.682472356046876 19.34806749772475 23 20.443163987596442 29.488567451175317 29.533597427558234 20.53467113367001 24 23.23245198288544 26.740357753733583 30.166108296843092 19.861081966537885 25 21.60927396709004 28.53892270896288 29.639223889137195 20.212579434809882 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 197.0 1 197.0 2 317.5 3 438.0 4 438.0 5 438.0 6 1124.0 7 1810.0 8 1810.0 9 1810.0 10 2419.5 11 3029.0 12 3029.0 13 3029.0 14 4918.0 15 6807.0 16 6807.0 17 6807.0 18 13419.5 19 20032.0 20 20032.0 21 20032.0 22 35102.5 23 50173.0 24 50173.0 25 50173.0 26 76566.5 27 102960.0 28 102960.0 29 102960.0 30 136108.5 31 169257.0 32 169257.0 33 169257.0 34 187530.0 35 205803.0 36 205803.0 37 205803.0 38 213275.5 39 220748.0 40 220748.0 41 220748.0 42 225808.0 43 230868.0 44 230868.0 45 230868.0 46 234154.0 47 237440.0 48 237440.0 49 237440.0 50 220392.0 51 203344.0 52 203344.0 53 203344.0 54 171635.5 55 139927.0 56 139927.0 57 139927.0 58 117236.5 59 94546.0 60 94546.0 61 94546.0 62 76589.0 63 58632.0 64 58632.0 65 58632.0 66 44257.5 67 29883.0 68 29883.0 69 29883.0 70 21581.5 71 13280.0 72 13280.0 73 13280.0 74 9119.0 75 4958.0 76 4958.0 77 4958.0 78 3317.5 79 1677.0 80 1677.0 81 1677.0 82 1108.5 83 540.0 84 540.0 85 540.0 86 367.0 87 194.0 88 194.0 89 194.0 90 122.5 91 51.0 92 51.0 93 51.0 94 26.5 95 2.0 96 2.0 97 2.0 98 2.5 99 3.0 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.16759443815787498 2 0.004508518595412778 3 0.0 4 0.001057553744602997 5 0.1172214834807322 6 0.03206057667849086 7 0.026104879274673982 8 0.02098409272185947 9 0.03495493429529906 10 0.03133698727428881 11 0.03645777382710332 12 0.03334077331669449 13 0.02476902191307019 14 0.007347215488820822 15 0.008627412127024451 16 0.0021707682126061523 17 6.679286808018929E-4 18 3.896250638011042E-4 19 5.566072340015775E-5 20 0.0 21 5.566072340015775E-5 22 0.005232107999614828 23 0.0010018930212028394 24 0.0 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1796599.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.58667121999223 #Duplication Level Percentage of deduplicated Percentage of total 1 83.38331968033317 53.854510534251865 2 11.414763791962725 14.744831921707366 3 2.692686815464273 5.217350341463967 4 0.9714007601912945 2.509581660853026 5 0.4417696918198679 1.426621692026355 6 0.25119864423272226 0.9734450547579978 7 0.15442305418240992 0.6981569720486449 8 0.11081258861903244 0.5725612982538961 9 0.07072414942903948 0.4111053647838277 >10 0.4284938412762237 5.119081043091913 >50 0.03847395922657646 1.7246613661434147 >100 0.03330556219571436 4.461243354434181 >500 0.00491765280814051 2.123060079102677 >1k 0.003278435205427007 4.144539136074592 >5k 4.3137305334565876E-4 2.019250181006347 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTGTAGGACGTGGAATATGGCAAGA 8373 0.4660472370295207 No Hit GTCCTACAGTGGACATTTCTAAATT 8234 0.45831039647689886 No Hit GTCCTAAAGTGTGTATTTCTCATTT 7814 0.43493289264883256 No Hit CTTTAGGACGTGAAATATGGCGAGG 6750 0.3757098829510648 No Hit GTATCAACGCAGAGTACTTTTTTTT 5067 0.2820328854685993 No Hit GTCCTACAGTGTGCATTTCTCATTT 4698 0.2614940785339411 No Hit CTGAAGGACCTGGAATATGGCGAGA 3929 0.21869098223921976 No Hit CTGTAGGACCTGGAATATGGCGAGA 3635 0.20232672955957343 No Hit GGTATCAACGCAGAGTACTTTTTTT 3484 0.19392196032614958 No Hit ATTTAGAAATGTCCACTGTAGGACG 3379 0.188077584369133 No Hit TATCAACGCAGAGTACTTTTTTTTT 3346 0.1862407804969278 No Hit GTCCTTCAGTGTGCATTTCTCATTT 3192 0.1776690290933035 No Hit TTTCTAAATTTTCCACCTTTTTCAG 2994 0.16664820586007228 No Hit GAATATGGCAAGAAAACTGAAAATC 2679 0.1491150779890226 No Hit GGAATATGGCGAGAAAACTGAAAAT 2364 0.1315819501179729 No Hit ATTCCAGGTCCTTCAGTGTGCATTT 2043 0.11371485790652226 No Hit GCCATATTCCACGTCCTACAGTGGA 1954 0.10876105352390823 No Hit TTCCAGGTCCTTCAGTGTGCATTTC 1856 0.10330630263069276 No Hit ACAGTGGACATTTCTAAATTTTCCA 1812 0.10085723080108583 No Hit GACCTGGAATATGGCGAGAAAACTG 1799 0.10013364139688377 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 5.566072340015774E-5 0.0 5 0.0 0.0 0.0 5.566072340015774E-5 0.0 6 5.566072340015774E-5 0.0 0.0 5.566072340015774E-5 0.0 7 5.566072340015774E-5 0.0 0.0 5.566072340015774E-5 0.0 8 5.566072340015774E-5 0.0 0.0 5.566072340015774E-5 0.0 9 5.566072340015774E-5 0.0 0.0 1.1132144680031548E-4 0.0 10 5.566072340015774E-5 0.0 0.0 1.1132144680031548E-4 0.0 11 5.566072340015774E-5 0.0 0.0 1.1132144680031548E-4 0.0 12 5.566072340015774E-5 0.0 0.0 1.1132144680031548E-4 0.0 13 5.566072340015774E-5 0.0 0.0 1.6698217020047322E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGTAGG 55 5.8046135E-7 17.30268 1 AATACCG 45 6.724366E-4 14.788962 5 GGTATCA 1160 0.0 13.946556 1 TAGGACC 1350 0.0 13.38049 4 TAGAAAT 1105 0.0 12.905688 4 CGTGCGC 60 4.100121E-4 12.663901 10 AGGCGTG 120 7.4396667E-10 12.663901 7 AAATGTC 1055 0.0 12.423826 7 AAGGCGT 115 5.340553E-9 12.388945 6 GCCGGTT 115 5.342372E-9 12.388599 11 CACCTTT 925 0.0 12.31889 14 TGTCGAG 210 0.0 12.207879 18 ACCTTTT 920 0.0 12.179409 15 TCCAACG 125 1.4224497E-9 12.153623 18 GTCCTAG 260 0.0 12.078601 1 GAAATGT 1135 0.0 11.632145 6 AATGTCC 1120 0.0 11.617998 8 GTCGAGG 180 0.0 11.605021 19 CCCCGGT 75 2.0842874E-4 11.394022 19 AGGACCT 2580 0.0 11.3865185 5 >>END_MODULE