FastQCFastQC Report
Thu 26 May 2016
SRR1512438_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512438_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1939175
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT69660.3592249281266518No Hit
GTCCTACAGTGGACATTTCTAAATT55410.2857400698750757No Hit
GTCCTAAAGTGTGTATTTCTCATTT50840.2621733469129914No Hit
CTGTAGGACGTGGAATATGGCAAGA48710.25118929441644No Hit
GGTATCAACGCAGAGTACTTTTTTT44500.22947903103123754No Hit
CTTTAGGACGTGAAATATGGCGAGG44190.22788041306225584No Hit
TATCAACGCAGAGTACTTTTTTTTT40390.20828445086183556No Hit
GTCCTACAGTGTGCATTTCTCATTT36880.19018396998723683No Hit
CTGTAGGACCTGGAATATGGCGAGA26250.1353668441476401No Hit
CTGAAGGACCTGGAATATGGCGAGA21950.11319246586821716No Hit
ACGCAGAGTACTTTTTTTTTTTTTT20700.10674642567071048No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCCGC400.005334065314.2280166
TAGGACC10300.013.5366334
AGGACGG802.0362677E-613.0416715
GGTATCA12650.012.7150021
GGCGAGG12400.011.54082119
TGATCCG752.1052785E-411.3818225
AGGACCT19900.011.0577155
GGACCTG19450.010.8752266
CCAACGA1053.5681278E-610.831120519
GTGTAGG3650.010.8140031
CTACCGT803.8561138E-410.66188410
TTAGGAC17900.010.5975993
TGTAGGA33300.010.5099682
CTGTAGG30350.010.436761
GACGTGA16700.010.3941197
GTCCTAC32050.010.3450031
AGGACGT31150.010.3221985
TGGCGAG32400.010.29438318
GGACGTG31250.010.28970056
GTATCAA28200.010.2526911
Produced by FastQC (version 0.11.5)