FastQCFastQC Report
Thu 26 May 2016
SRR1512438_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512438_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1939175
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT83710.4316784199466268No Hit
GTCCTACAGTGGACATTTCTAAATT59190.3052328954323359No Hit
GGTATCAACGCAGAGTACTTTTTTT58310.30069488313329124No Hit
TATCAACGCAGAGTACTTTTTTTTT56800.29290806657470314No Hit
CTGTAGGACGTGGAATATGGCAAGA56280.2902265138525404No Hit
GTCCTAAAGTGTGTATTTCTCATTT53730.2770765918496268No Hit
CTTTAGGACGTGAAATATGGCGAGG52790.2722291696211018No Hit
GTCCTACAGTGTGCATTTCTCATTT38930.20075547591114776No Hit
CTGTAGGACCTGGAATATGGCGAGA30970.1597070919334253No Hit
ACGCAGAGTACTTTTTTTTTTTTTT26370.13598566400660073No Hit
CTGAAGGACCTGGAATATGGCGAGA26220.13521213918289995No Hit
TTTCTAAATTTTCCACCTTTTTCAG21320.10994366160867379No Hit
GTACTTTTTTTTTTTTTTTTTTTTT20680.10664328902755037No Hit
ATTTAGAAATGTCCACTGTAGGACG20640.10643701574123016No Hit
GAATATGGCAAGAAAACTGAAAATC20500.1057150592391094No Hit
GTCCTTCAGTGTGCATTTCTCATTT19890.10256939162272616No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCAATA250.00600601719.0145342
CTGCGAT602.5735166E-514.2472929
GGTATCA15500.013.8758241
TAGGACC12350.013.4707944
TCCAACG1800.012.66066718
TCTATAC2350.012.1359983
CGCCCTA2600.012.05198116
ACGAAAT1701.8189894E-1211.73215712
TACACCG657.977127E-411.7006485
TGGCGAA907.5025728E-611.60561218
CCAACGA1406.85759E-1011.5302519
GTCCTAG3450.011.30958751
AGGACCT21750.011.2770045
TCCCGTT1354.7439244E-911.2571211
TCCCGCC2700.011.25537813
TGTAGGA35100.011.1053552
GGACCTG21150.011.0924536
AATGTCC8050.010.9730698
CTGTAGG35000.010.903311
GTAGGAC35400.010.8761173
Produced by FastQC (version 0.11.5)