##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512438_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1939175 Sequences flagged as poor quality 0 Sequence length 25 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.884006342903554 34.0 31.0 34.0 30.0 34.0 2 32.051087189139714 34.0 31.0 34.0 30.0 34.0 3 32.17739038508644 34.0 31.0 34.0 30.0 34.0 4 35.61922518596826 37.0 35.0 37.0 33.0 37.0 5 35.42815578789949 37.0 35.0 37.0 33.0 37.0 6 35.45727951319505 37.0 35.0 37.0 33.0 37.0 7 35.37114082019416 37.0 35.0 37.0 33.0 37.0 8 35.334659326775565 37.0 35.0 37.0 33.0 37.0 9 36.983375920172236 39.0 37.0 39.0 33.0 39.0 10 36.910911083321515 39.0 37.0 39.0 33.0 39.0 11 36.951012157231816 39.0 37.0 39.0 33.0 39.0 12 36.87866747457037 39.0 37.0 39.0 33.0 39.0 13 36.85835471269999 39.0 37.0 39.0 33.0 39.0 14 38.17788337824074 40.0 38.0 41.0 33.0 41.0 15 38.175966583727615 40.0 38.0 41.0 33.0 41.0 16 38.13051735918625 40.0 38.0 41.0 33.0 41.0 17 38.128800649760855 40.0 38.0 41.0 33.0 41.0 18 38.07851689507136 40.0 38.0 41.0 33.0 41.0 19 38.10624569726817 40.0 38.0 41.0 33.0 41.0 20 38.09105779519641 40.0 38.0 41.0 33.0 41.0 21 38.04436783683783 40.0 38.0 41.0 33.0 41.0 22 37.98596052444983 40.0 38.0 41.0 33.0 41.0 23 37.91028504389753 40.0 38.0 41.0 33.0 41.0 24 37.86463624995165 40.0 38.0 41.0 33.0 41.0 25 37.80530122345843 40.0 38.0 41.0 33.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.4357931661024814 1101 2 0.45778803935756684 1101 3 0.5070345407011914 1101 4 0.3610695603733518 1101 5 0.33631802091230867 1101 6 0.5012747872509848 1101 7 0.5411179344227577 1101 8 0.5805878759298579 1101 9 0.740298200033962 1101 10 0.7011611146507235 1101 11 0.7668927794088773 1101 12 0.7587822692198358 1101 13 0.8005068320081961 1101 14 0.9230333789119669 1101 15 0.8148494130104709 1101 16 0.8026296537259512 1101 17 0.7902218279019735 1101 18 0.8106363357585167 1101 19 0.7586103448974626 1101 20 0.6185892949490395 1101 21 0.7959430073935323 1101 22 0.7925454119932951 1101 23 0.8648948176984916 1101 24 0.9715706667911377 1101 25 0.7218812994568395 1102 1 0.5816617019560475 1102 2 0.5327354095820667 1102 3 0.526755983024735 1102 4 0.4182234620440113 1102 5 0.3646294135721959 1102 6 0.44453046623398507 1102 7 0.5093885510531209 1102 8 0.5432908726682015 1102 9 0.6643469556583383 1102 10 0.6675080407077587 1102 11 0.6870839812951957 1102 12 0.7254670323912009 1102 13 0.810000536780322 1102 14 0.8397091473709324 1102 15 0.9410120331273575 1102 16 0.8379612052495915 1102 17 0.8162277901113342 1102 18 0.7664048375606711 1102 19 0.8545871385394861 1102 20 0.854833809780672 1102 21 0.8942327270797463 1102 22 0.8649501697678375 1102 23 0.8919341010340744 1102 24 0.9780447181166778 1102 25 1.0149675780230822 1103 1 0.4420213879466992 1103 2 0.47695333520329086 1103 3 0.47188497985671063 1103 4 0.3159232521484725 1103 5 0.4179375038443567 1103 6 0.3804643506477561 1103 7 0.5132079163025054 1103 8 0.43236776436552304 1103 9 0.5700479454529344 1103 10 0.5640935457582117 1103 11 0.6143309134946193 1103 12 0.5572378714810071 1103 13 0.6207298257981364 1103 14 0.7585276033074422 1103 15 0.9340063049586504 1103 16 0.7531913920254709 1103 17 0.8120120448314694 1103 18 0.8181889496040924 1103 19 0.7635779821105402 1103 20 0.7997407534143264 1103 21 0.7915994373867363 1103 22 0.6793586592333725 1103 23 0.7333572208915626 1103 24 0.7922605437777861 1103 25 0.9116475310402379 1104 1 0.3665137469369064 1104 2 0.36928453904865677 1104 3 0.33682358111328625 1104 4 0.22057235693809218 1104 5 0.25230702695550633 1104 6 0.1974695566315674 1104 7 0.3043228549037593 1104 8 0.33122564407889143 1104 9 0.3360375814755727 1104 10 0.34192477935754084 1104 11 0.3441752426771174 1104 12 0.3171920119849787 1104 13 0.3249783078660897 1104 14 0.312641184744038 1104 15 0.40020652225856423 1104 16 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2315 13 -0.9612307182543702 2315 14 -1.3668261322377901 2315 15 -1.4146550654017602 2315 16 -1.4489759299634741 2315 17 -1.389135485876281 2315 18 -1.3073159741114821 2315 19 -1.2232878059015704 2315 20 -1.1393960121125986 2315 21 -1.0985125855505515 2315 22 -1.1087415406223968 2315 23 -1.2553607263312756 2315 24 -1.4148458000236914 2315 25 -1.3878749767341816 2316 1 -1.2841637251264544 2316 2 -1.236181971606733 2316 3 -1.0948510938247438 2316 4 -0.899777422199783 2316 5 -0.9943326146801184 2316 6 -1.124306825660689 2316 7 -0.9813214532231385 2316 8 -1.0493409122721076 2316 9 -1.3614051951559674 2316 10 -1.1562027390937928 2316 11 -1.3607159186837805 2316 12 -1.4215613286411894 2316 13 -1.309789129163967 2316 14 -1.6254748405685575 2316 15 -1.5209024271577718 2316 16 -1.768997141069562 2316 17 -1.7963426051166493 2316 18 -1.9951182361287252 2316 19 -2.0699020657135847 2316 20 -1.843403138563545 2316 21 -1.831773462626785 2316 22 -1.902677447630026 2316 23 -2.0408021261441007 2316 24 -1.8457450637940127 2316 25 -1.915403297015878 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 13864.0 3 0.0 4 276.0 5 2267.0 6 3078.0 7 2477.0 8 1881.0 9 1722.0 10 1602.0 11 1518.0 12 1482.0 13 1480.0 14 1466.0 15 1413.0 16 1543.0 17 1621.0 18 1804.0 19 2052.0 20 2545.0 21 3368.0 22 4432.0 23 5439.0 24 6686.0 25 8033.0 26 9114.0 27 10322.0 28 12453.0 29 14885.0 30 18988.0 31 25474.0 32 33260.0 33 44094.0 34 59899.0 35 83101.0 36 124968.0 37 237250.0 38 1193318.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.6702479338843 20.0834194214876 13.471229338842974 24.775103305785123 2 14.277794452403569 20.923286063347764 42.523854169567535 22.27506531468113 3 18.399113024868825 25.653486663142832 30.575579821315767 25.37182049067258 4 12.073836093978835 17.820190185441117 39.27484065265372 30.83113306792632 5 12.14129357343466 38.791508342970424 36.581705352717655 12.485492730877251 6 30.40915913979604 38.50109098787276 18.551900588566035 12.537849283765171 7 27.030956879481348 31.987606313208296 23.12468632800149 17.856750479308868 8 23.785294328510943 38.67391374987557 21.0806358356161 16.46015608599739 9 26.118476777259986 14.930923451980682 21.169220261903853 37.78137950885548 10 14.669540852434393 29.39097385997165 35.458332559593444 20.481152728000513 11 33.225382954741825 22.208099373499195 26.402224348673876 18.164293323085097 12 23.370815844274688 24.908580604593126 32.969478379422604 18.751125171709585 13 29.086351362852124 22.689753528663868 26.2568181290667 21.96707697941731 14 19.671170736763827 23.792082868976273 28.483941454136826 28.05280494012308 15 21.715099967818862 33.41315012088556 23.76613182714603 21.10561808414955 16 20.467805798246438 28.661601185681473 29.994513025890885 20.876079990181204 17 20.376985446888956 29.795845961139104 29.103437393156774 20.723731198815166 18 20.061438561696146 27.376979774367733 32.6934729392375 19.868108724698622 19 22.81021568450501 26.96254850645249 28.837985225675865 21.389250583366636 20 23.047223690486934 27.648097773537717 29.7212990060206 19.583379529954748 21 21.677930047571778 27.019944048371087 28.466848015264222 22.835277888792916 22 22.394679178319414 29.32547538831179 28.559449517533658 19.720395915835137 23 20.737905612934235 28.982747204465433 29.58898783186982 20.69035935073052 24 22.512357059058623 27.080021143011844 30.358167777534263 20.04945402039527 25 21.46938775510204 28.27408562919798 29.794680727629018 20.461845888070957 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 242.0 1 242.0 2 472.5 3 703.0 4 703.0 5 703.0 6 1878.5 7 3054.0 8 3054.0 9 3054.0 10 4078.5 11 5103.0 12 5103.0 13 5103.0 14 7769.0 15 10435.0 16 10435.0 17 10435.0 18 18807.5 19 27180.0 20 27180.0 21 27180.0 22 43462.0 23 59744.0 24 59744.0 25 59744.0 26 87458.0 27 115172.0 28 115172.0 29 115172.0 30 147585.5 31 179999.0 32 179999.0 33 179999.0 34 198469.0 35 216939.0 36 216939.0 37 216939.0 38 225020.0 39 233101.0 40 233101.0 41 233101.0 42 239161.5 43 245222.0 44 245222.0 45 245222.0 46 247116.5 47 249011.0 48 249011.0 49 249011.0 50 232826.5 51 216642.0 52 216642.0 53 216642.0 54 185380.5 55 154119.0 56 154119.0 57 154119.0 58 128944.0 59 103769.0 60 103769.0 61 103769.0 62 83691.0 63 63613.0 64 63613.0 65 63613.0 66 48216.0 67 32819.0 68 32819.0 69 32819.0 70 23554.0 71 14289.0 72 14289.0 73 14289.0 74 9855.5 75 5422.0 76 5422.0 77 5422.0 78 3577.5 79 1733.0 80 1733.0 81 1733.0 82 1187.5 83 642.0 84 642.0 85 642.0 86 402.0 87 162.0 88 162.0 89 162.0 90 107.5 91 53.0 92 53.0 93 53.0 94 28.0 95 3.0 96 3.0 97 3.0 98 3.5 99 4.0 100 4.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.16372942101666946 2 0.005208400479585391 3 0.0 4 7.735248237008005E-4 5 0.11422383229981822 6 0.029136101692730156 7 0.022999471424703804 8 0.019183415627779855 9 0.030992561269612078 10 0.02779532533164877 11 0.03248804259543363 12 0.030012763159591064 13 0.021143011847821882 14 0.005878788660126084 15 0.00778681655858806 16 0.0019080278984619748 17 1.0313664316010674E-4 18 1.0313664316010674E-4 19 0.0 20 0.0 21 0.0 22 0.003919192440084056 23 5.672515373805871E-4 24 0.0 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1939175.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.01176262566938 #Duplication Level Percentage of deduplicated Percentage of total 1 83.93054037150948 57.08263988791262 2 11.281338132787699 15.345273823741385 3 2.559708525523395 5.222708658863979 4 0.8632489140909895 2.348443209280929 5 0.397347511439646 1.3512152313966825 6 0.21917547387599892 0.8943906181573822 7 0.13567300994732243 0.645915238307316 8 0.09908949211013385 0.539140081287405 9 0.07873085024378336 0.4819161508287586 >10 0.3699228036856261 4.644659645638597 >50 0.032865717525505184 1.562466541630841 >100 0.02651164811563404 3.7725262824162513 >500 0.003189572260847321 1.4936039003812662 >1k 0.002126381507231541 2.441632795584876 >5k 5.315953768078853E-4 2.1734679345716827 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 8371 0.4316784199466268 No Hit GTCCTACAGTGGACATTTCTAAATT 5919 0.3052328954323359 No Hit GGTATCAACGCAGAGTACTTTTTTT 5831 0.30069488313329124 No Hit TATCAACGCAGAGTACTTTTTTTTT 5680 0.29290806657470314 No Hit CTGTAGGACGTGGAATATGGCAAGA 5628 0.2902265138525404 No Hit GTCCTAAAGTGTGTATTTCTCATTT 5373 0.2770765918496268 No Hit CTTTAGGACGTGAAATATGGCGAGG 5279 0.2722291696211018 No Hit GTCCTACAGTGTGCATTTCTCATTT 3893 0.20075547591114776 No Hit CTGTAGGACCTGGAATATGGCGAGA 3097 0.1597070919334253 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 2637 0.13598566400660073 No Hit CTGAAGGACCTGGAATATGGCGAGA 2622 0.13521213918289995 No Hit TTTCTAAATTTTCCACCTTTTTCAG 2132 0.10994366160867379 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 2068 0.10664328902755037 No Hit ATTTAGAAATGTCCACTGTAGGACG 2064 0.10643701574123016 No Hit GAATATGGCAAGAAAACTGAAAATC 2050 0.1057150592391094 No Hit GTCCTTCAGTGTGCATTTCTCATTT 1989 0.10256939162272616 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 5.1568321580053376E-5 0.0 5 0.0 0.0 0.0 5.1568321580053376E-5 0.0 6 0.0 0.0 0.0 5.1568321580053376E-5 0.0 7 0.0 0.0 0.0 5.1568321580053376E-5 0.0 8 0.0 0.0 0.0 5.1568321580053376E-5 0.0 9 0.0 0.0 0.0 5.1568321580053376E-5 0.0 10 0.0 0.0 0.0 5.1568321580053376E-5 0.0 11 0.0 0.0 0.0 5.1568321580053376E-5 0.0 12 0.0 0.0 0.0 1.0313664316010675E-4 0.0 13 0.0 0.0 0.0 1.0313664316010675E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCAATA 25 0.006006017 19.014534 2 CTGCGAT 60 2.5735166E-5 14.247292 9 GGTATCA 1550 0.0 13.875824 1 TAGGACC 1235 0.0 13.470794 4 TCCAACG 180 0.0 12.660667 18 TCTATAC 235 0.0 12.135998 3 CGCCCTA 260 0.0 12.051981 16 ACGAAAT 170 1.8189894E-12 11.732157 12 TACACCG 65 7.977127E-4 11.700648 5 TGGCGAA 90 7.5025728E-6 11.605612 18 CCAACGA 140 6.85759E-10 11.53025 19 GTCCTAG 345 0.0 11.3095875 1 AGGACCT 2175 0.0 11.277004 5 TCCCGTT 135 4.7439244E-9 11.25712 11 TCCCGCC 270 0.0 11.255378 13 TGTAGGA 3510 0.0 11.105355 2 GGACCTG 2115 0.0 11.092453 6 AATGTCC 805 0.0 10.973069 8 CTGTAGG 3500 0.0 10.90331 1 GTAGGAC 3540 0.0 10.876117 3 >>END_MODULE