FastQCFastQC Report
Thu 26 May 2016
SRR1512437_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512437_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2182333
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT76920.3524668325136448No Hit
GTCCTAAAGTGTGTATTTCTCATTT70690.3239194018511382No Hit
CTGTAGGACGTGGAATATGGCAAGA69710.3194287947806316No Hit
GTATCAACGCAGAGTACTTTTTTTT66240.3035283799493478No Hit
CTTTAGGACGTGAAATATGGCGAGG59350.2719566628924183No Hit
GGTATCAACGCAGAGTACTTTTTTT42340.19401255445433854No Hit
GTCCTACAGTGTGCATTTCTCATTT41350.1894761248627043No Hit
TATCAACGCAGAGTACTTTTTTTTT37360.1711929389327843No Hit
CTGAAGGACCTGGAATATGGCGAGA33080.1515808998901634No Hit
CTGTAGGACCTGGAATATGGCGAGA29820.13664275800255965No Hit
GTCCTTCAGTGTGCATTTCTCATTT28400.13013596000243777No Hit
ATTTAGAAATGTCCACTGTAGGACG25920.11877197476278827No Hit
GAATATGGCAAGAAAACTGAAAATC25160.11528946315708921No Hit
TTTCTAAATTTTCCACCTTTTTCAG22890.10488775086111973No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC13300.013.3354494
GGTATCA13050.013.0832631
CCAACGA1550.012.83616219
GTGTAGG4300.012.6233531
TCCAACG2450.011.98514318
GTCCTAT3300.011.9626261
GGCGAGG17150.011.71168219
CGTAGGT658.1736565E-411.66686610
TGTCGAG2200.011.62488618
GTCCTAA21600.011.4683381
GGACCTG27350.011.4439076
AATACGG752.09754E-411.38647114
CCACCTT10150.011.21483613
ACCTTTT10500.011.20573415
AGGACCT28050.011.1921325
GTCCTAC39600.011.1152741
CACCTTT10700.010.81892114
CCGAGCC700.001548894810.8083759
ATGTCGA2200.010.78164917
GCCTAAG1654.783942E-1010.7663631
Produced by FastQC (version 0.11.5)