FastQCFastQC Report
Thu 26 May 2016
SRR1512437_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512437_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2182333
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT83990.3848633549508714No Hit
GTCCTACAGTGGACATTTCTAAATT83120.38087679561276855No Hit
CTGTAGGACGTGGAATATGGCAAGA81350.37276620937317995No Hit
GTCCTAAAGTGTGTATTTCTCATTT74720.3423858778655686No Hit
CTTTAGGACGTGAAATATGGCGAGG71270.3265771080765401No Hit
GGTATCAACGCAGAGTACTTTTTTT58420.26769516842754976No Hit
TATCAACGCAGAGTACTTTTTTTTT55930.25628536066677265No Hit
GTCCTACAGTGTGCATTTCTCATTT44770.20514742708834993No Hit
CTGAAGGACCTGGAATATGGCGAGA39430.18067820080620145No Hit
CTGTAGGACCTGGAATATGGCGAGA36130.16555676883408718No Hit
GTCCTTCAGTGTGCATTTCTCATTT29290.13421416438279585No Hit
ATTTAGAAATGTCCACTGTAGGACG29150.13357264908700917No Hit
TTTCTAAATTTTCCACCTTTTTCAG28880.13233544101656347No Hit
ACGCAGAGTACTTTTTTTTTTTTTT28080.12866963932635395No Hit
GAATATGGCAAGAAAACTGAAAATC26840.1229876467065292No Hit
GGAATATGGCGAGAAAACTGAAAAT23440.10740798952313876No Hit
ATTCCAGGTCCTTCAGTGTGCATTT22730.10415459052307782No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14650.013.5092931
TAGGACC12600.013.20374
TCCAACG2850.012.99302318
AGGACCG751.4686037E-512.6755535
CCTACAC3200.012.1804123
TTCGCAT550.003081491212.08442117
GTGGTAT5050.012.0585621
CCAACGA2100.011.75559219
ACCTTTT10500.011.30449115
AGGACCT24800.011.2699975
CACCTTT10250.011.20964314
GACGTGT600.00588238611.0819577
GGACCTG24450.011.0336476
ACGGTAC700.001483118610.86475853
GCCGGTT1752.0008883E-1110.85529611
TAGAAAT11100.010.7913484
AAATGTC10250.010.7498847
CCACCTT10800.010.64000113
AATGTCC10650.010.6137048
GGCGAGG18950.010.4719519
Produced by FastQC (version 0.11.5)