FastQCFastQC Report
Thu 26 May 2016
SRR1512436_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512436_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1469747
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT51710.351829260410125No Hit
GTCCTACAGTGGACATTTCTAAATT42900.29188697102290395No Hit
CTGTAGGACGTGGAATATGGCAAGA40290.274128812645986No Hit
GTCCTAAAGTGTGTATTTCTCATTT39420.26820942652034674No Hit
GGTATCAACGCAGAGTACTTTTTTT32840.2234398165126379No Hit
CTTTAGGACGTGAAATATGGCGAGG32760.222895505144763No Hit
TATCAACGCAGAGTACTTTTTTTTT29510.20078285582484606No Hit
GTCCTACAGTGTGCATTTCTCATTT24510.1667633953326661No Hit
CTGAAGGACCTGGAATATGGCGAGA18040.12274221345578525No Hit
CTGTAGGACCTGGAATATGGCGAGA15650.10648091134052323No Hit
ACGCAGAGTACTTTTTTTTTTTTTT15530.10566444428871091No Hit
ATTTAGAAATGTCCACTGTAGGACG15510.1055283664467422No Hit
GTCCTTCAGTGTGCATTTCTCATTT15270.10389543234311756No Hit
GTACTGGTTCACTATCGGTCAGTCA15010.1021264203975242No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCCAC551.985235E-413.79737813
GGTATCA9000.013.5972721
TAGGACC7600.013.47829154
GTCTTAC1351.9645086E-1012.4276141
AATGTCC5350.011.8371588
GTGTAGG2500.011.4480261
GTTCTAA1900.011.4272491
TAGAAAT6000.011.2235894
AGGACCT15250.011.1950835
TGTTAGG1201.3034332E-711.0655112
GAAATGT6300.010.9902356
TGTAGGA24550.010.8562412
GGCGAGG9700.010.84474719
ATAAGAC1408.558345E-910.8396843
GTGGTAT3600.010.6914041
GTCCTAC20800.010.6755871
TAGGGTC803.8268665E-410.6703145
AAATGTC6050.010.6605167
TGTCCAC6600.010.62747810
GTCCTAA11000.010.5867321
Produced by FastQC (version 0.11.5)