FastQCFastQC Report
Thu 26 May 2016
SRR1512436_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512436_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1469747
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT64300.43749026192943413No Hit
CTGTAGGACGTGGAATATGGCAAGA46210.3144078538687271No Hit
GTCCTACAGTGGACATTTCTAAATT45220.3076720006912754No Hit
GGTATCAACGCAGAGTACTTTTTTT43900.2986908631213399No Hit
GTCCTAAAGTGTGTATTTCTCATTT41470.28215740532214045No Hit
TATCAACGCAGAGTACTTTTTTTTT39220.2668486481006595No Hit
CTTTAGGACGTGAAATATGGCGAGG37920.2580035883726927No Hit
GTCCTACAGTGTGCATTTCTCATTT26100.17758158376917932No Hit
CTGAAGGACCTGGAATATGGCGAGA21660.14737230285212352No Hit
ACGCAGAGTACTTTTTTTTTTTTTT20610.14022821614876574No Hit
CTGTAGGACCTGGAATATGGCGAGA19250.1309749228948928No Hit
ATTTAGAAATGTCCACTGTAGGACG16620.11308068667600614No Hit
TTTCTAAATTTTCCACCTTTTTCAG16180.1100869741526943No Hit
GTACTGGTTCACTATCGGTCAGTCA15780.1073654173133199No Hit
GTCCTTCAGTGTGCATTTCTCATTT15750.10716130055036684No Hit
GAATATGGCAAGAAAACTGAAAATC15390.10471189939492988No Hit
GTACTTTTTTTTTTTTTTTTTTTTT14790.1006295641358683No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12050.013.8192841
TAGGACC7950.013.1549774
TCCAACG1052.737288E-711.75592318
ACCTTTT5050.011.46980215
AGGACCT15100.011.2704325
GGACCTG14650.010.95726
GTGCGCC700.001494262610.85457611
TAGAAAT6500.010.8238784
GGCGAGG9950.010.78345919
TAGGACA5750.010.7475644
TAGACAG2400.010.6958925
TGTAGGA25300.010.6720432
GATATAC2950.010.6445281
CTAGGCT1701.364242E-1010.6259844
AATCCCG1553.6907295E-910.41405619
CACCTTT5500.010.35871914
TTTAGAA7550.010.3256312
TACACTG7600.010.2580495
GTAGGAC25550.010.2330783
GAAATGT6500.010.2290476
Produced by FastQC (version 0.11.5)