FastQCFastQC Report
Thu 26 May 2016
SRR1512435_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512435_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1851847
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT84090.45408718970843703No Hit
GGTATCAACGCAGAGTACTTTTTTT52650.28431074489415165No Hit
TATCAACGCAGAGTACTTTTTTTTT45010.24305463680314843No Hit
GTCCTACAGTGGACATTTCTAAATT29150.15741041241528053No Hit
CTGTAGGACGTGGAATATGGCAAGA24750.13365035016391744No Hit
GTCCTAAAGTGTGTATTTCTCATTT22480.12139231804787329No Hit
ACGCAGAGTACTTTTTTTTTTTTTT21930.11842231026645289No Hit
CTTTAGGACGTGAAATATGGCGAGG20430.1103222890443973No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12550.014.7924261
ATCGCGC707.654848E-613.5027838
TCGCGCG655.691713E-513.0940479
TAGGACC6350.012.9942254
TATCGCG604.1501957E-412.64572057
CGCGCCT658.173975E-411.66663612
TGTAGGA17150.011.3922082
CTTATAC1950.011.1865983
GTGCGCC855.450411E-511.15072111
GGCGAGG7200.011.05629819
GTAGGAC16900.011.0556523
AGGACGT16650.010.9938015
ATAGGAC2850.010.981813
CTGTAGG15600.010.9508241
TAGGACG17400.010.8469754
GGACGTG16450.010.839196
TGGTATC3500.010.8391892
CGTGCGC700.00152129410.83154110
TTAGGAC10450.010.8002933
ATGTCGA803.816069E-410.67388417
Produced by FastQC (version 0.11.5)