FastQCFastQC Report
Thu 26 May 2016
SRR1512435_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512435_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1851847
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT104160.562465473659541No Hit
GGTATCAACGCAGAGTACTTTTTTT68520.3700089694234999No Hit
TATCAACGCAGAGTACTTTTTTTTT63130.3409028931655801No Hit
ACGCAGAGTACTTTTTTTTTTTTTT30070.16237842543147463No Hit
CTGTAGGACGTGGAATATGGCAAGA30070.16237842543147463No Hit
GTCCTACAGTGGACATTTCTAAATT29310.15827441467896644No Hit
CTTTAGGACGTGAAATATGGCGAGG24190.13062634224101669No Hit
GTCCTAAAGTGTGTATTTCTCATTT23780.12841233644032146No Hit
GTACTTTTTTTTTTTTTTTTTTTTT19200.10368027164231171No Hit
GAGTACTTTTTTTTTTTTTTTTTTT18650.10071026386089131No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15850.013.6888341
TAGGACC6400.012.9237654
CCAACGA1155.3641998E-912.38521119
TCCAACG1701.8189894E-1211.72952318
CTAGGAC2650.011.1215573
ATAGGAC2150.011.0548113
GGCGAGG6550.011.01748119
CGAACCG1053.4782515E-610.85414912
AGGACCT11350.010.8054695
CTGCTCG803.778201E-410.6854189
GGACCTG11050.010.40096956
TGGTATC4800.010.101062
GTGGTAT5600.010.0259351
TGGCGAG16350.09.9889718
CAGAACG1252.5454392E-69.8874234
CAAGTCG1356.457449E-79.85026
GTATCAA32150.09.8269331
TGTAGGA18750.09.6843412
GATATAC4100.09.5161421
CGGTACT800.00445332569.5161421
Produced by FastQC (version 0.11.5)