FastQCFastQC Report
Thu 26 May 2016
SRR1512434_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512434_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2602721
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT82590.3173217567307445No Hit
CTGTAGGACGTGGAATATGGCAAGA76000.29200210087827316No Hit
GTATCAACGCAGAGTACTTTTTTTT73120.2809367581081491No Hit
GTCCTAAAGTGTGTATTTCTCATTT72280.27770936646686295No Hit
CTTTAGGACGTGAAATATGGCGAGG62790.24124752518614173No Hit
GTCCTACAGTGTGCATTTCTCATTT47840.18380763823706037No Hit
GGTATCAACGCAGAGTACTTTTTTT47370.18200183577110263No Hit
TATCAACGCAGAGTACTTTTTTTTT45160.17351072204819493No Hit
CTGTAGGACCTGGAATATGGCGAGA35830.13766362203248061No Hit
CTGAAGGACCTGGAATATGGCGAGA32900.12640617261704193No Hit
GTCCTTCAGTGTGCATTTCTCATTT28460.1093471025131007No Hit
ATTTAGAAATGTCCACTGTAGGACG26630.1023159992945844No Hit
GAATATGGCAAGAAAACTGAAAATC26170.1005486181576896No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACGTA456.8515225E-414.7528766
TAGGACC14150.013.5392464
GGTATCA14150.013.5257631
GTCGAGG2150.012.78173919
GGCGAGG17850.011.89159919
TCCAACG1157.363451E-811.5325818
GTGTAGG5950.011.2747881
TATGTCG2550.011.1645416
TTACACG855.417003E-511.1578524
CCAACGA855.4640914E-511.14837419
TGTCGAG2900.011.106518
TGTAGGA47850.011.0004532
AGGACCT30700.010.9979355
CTAGGAC4100.010.8721013
CACCTTT11000.010.87041214
GTCCTAG3850.010.7622981
GTCCTAA23250.010.7352961
CCACCTT11100.010.68244113
GGACCTG29950.010.6714686
CGAGTAA803.8509435E-410.6637210
Produced by FastQC (version 0.11.5)