FastQCFastQC Report
Thu 26 May 2016
SRR1512434_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512434_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2602721
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT94680.3637731435678277No Hit
CTGTAGGACGTGGAATATGGCAAGA88260.33910665030942616No Hit
GTCCTACAGTGGACATTTCTAAATT87770.33722400518534257No Hit
GTCCTAAAGTGTGTATTTCTCATTT78150.3002626866268033No Hit
CTTTAGGACGTGAAATATGGCGAGG76160.29261684214328004No Hit
GGTATCAACGCAGAGTACTTTTTTT63910.24555071404118994No Hit
TATCAACGCAGAGTACTTTTTTTTT63300.24320701296835118No Hit
GTCCTACAGTGTGCATTTCTCATTT52080.200098281759743No Hit
CTGTAGGACCTGGAATATGGCGAGA41580.15975588624366577No Hit
CTGAAGGACCTGGAATATGGCGAGA41070.1577963984614563No Hit
ATTTAGAAATGTCCACTGTAGGACG29810.11453398193659635No Hit
GTCCTTCAGTGTGCATTTCTCATTT29780.11441871794940757No Hit
ACGCAGAGTACTTTTTTTTTTTTTT29630.11384239801346359No Hit
GAATATGGCAAGAAAACTGAAAATC29530.1134581847228343No Hit
TTTCTAAATTTTCCACCTTTTTCAG29500.1133429207356455No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGTTT1101.2732926E-1114.678966512
GGTATCA15700.013.9372631
GCCGGTT1400.013.56957611
TAGGACC15700.013.4434314
CGAACCG1501.8189894E-1212.66420712
GCGAACC1457.2759576E-1212.44657511
CGTTGAA550.003071657612.0897239
GGCGAGG20100.012.04526819
TGTCGAG1750.011.9359518
TCCAACG2100.011.75510218
GCACCGT658.031925E-411.6913856
TGTAGGA52350.011.223292
AGAACCG855.283334E-511.1852645
AGGACCT29200.011.1354665
TGCACCG600.00584315711.0920535
CGTAGTA600.005844830511.0916262
GGCGAAC1900.010.99870910
GTAGGAC52250.010.9720523
CTGTAGG50850.010.9262511
GCGTGCG700.001493209310.8560789
Produced by FastQC (version 0.11.5)