FastQCFastQC Report
Thu 26 May 2016
SRR1512433_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512433_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1834888
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT79730.43452243406682045No Hit
GTCCTACAGTGGACATTTCTAAATT61450.33489782482636543No Hit
GTCCTAAAGTGTGTATTTCTCATTT59210.32268999524766634No Hit
CTGTAGGACGTGGAATATGGCAAGA56210.30634022349048007No Hit
GGTATCAACGCAGAGTACTTTTTTT48400.2637763176826052No Hit
CTTTAGGACGTGAAATATGGCGAGG48070.2619778427893147No Hit
TATCAACGCAGAGTACTTTTTTTTT46690.25445694778100897No Hit
GTCCTACAGTGTGCATTTCTCATTT32320.17614154106408675No Hit
CTGAAGGACCTGGAATATGGCGAGA22910.12485775698571248No Hit
GTACTGGTTCACTATCGGTCAGTCA22900.12480325774652185No Hit
ACGCAGAGTACTTTTTTTTTTTTTT22790.12420376611542504No Hit
GTCCTTCAGTGTGCATTTCTCATTT22060.1202253216545097No Hit
ATTTAGAAATGTCCACTGTAGGACG21790.11875384219636294No Hit
CTGTAGGACCTGGAATATGGCGAGA20980.11433940382192265No Hit
GTACTTTTTTTTTTTTTTTTTTTTT19080.10398454837570467No Hit
GAATATGGCAAGAAAACTGAAAATC18500.10082359250264868No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC8650.013.3781544
GGTATCA11950.012.6389211
TTGCGAG751.5187692E-512.633944518
GGTCTAG600.00441469211.5168391
TAGAACG752.1041709E-411.3823984
AATCCCG1251.8964784E-811.37335419
GTATCAA27900.011.180711
AGAGCGT600.00594429411.0662196
GGCGAGG15000.010.99424219
TCCAACG1303.3933247E-810.9332218
TGTAGGA34950.010.9101672
AGGACCT18900.010.8905655
CCTAGAC1408.556526E-910.8403783
GTATAGG1551.9444997E-910.826891
CCACCTT6700.010.75918513
GTATAAT2300.010.7299741
AGGACGT36550.010.640185
GTCCTAT1302.2665517E-710.6309271
ATCCTAC3350.010.608261
CTGTAGG31650.010.6045361
Produced by FastQC (version 0.11.5)