FastQCFastQC Report
Thu 26 May 2016
SRR1512433_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512433_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1834888
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT99980.5448833934278278No Hit
TATCAACGCAGAGTACTTTTTTTTT66300.36132995583381655No Hit
GGTATCAACGCAGAGTACTTTTTTT66230.3609484611594822No Hit
CTGTAGGACGTGGAATATGGCAAGA66040.3599129756148604No Hit
GTCCTACAGTGGACATTTCTAAATT65280.35577103343637323No Hit
GTCCTAAAGTGTGTATTTCTCATTT60720.3309193803654501No Hit
CTTTAGGACGTGAAATATGGCGAGG56820.30966467708110795No Hit
GTCCTACAGTGTGCATTTCTCATTT33640.18333544063724871No Hit
ACGCAGAGTACTTTTTTTTTTTTTT31410.17118211029774025No Hit
CTGAAGGACCTGGAATATGGCGAGA28210.15374235375674156No Hit
CTGTAGGACCTGGAATATGGCGAGA26430.14404148918081103No Hit
ATTTAGAAATGTCCACTGTAGGACG25490.13891856069689268No Hit
GTACTGGTTCACTATCGGTCAGTCA23700.12916319688177155No Hit
GTACTTTTTTTTTTTTTTTTTTTTT23040.12556624709519057No Hit
GTCCTTCAGTGTGCATTTCTCATTT23000.12534825013842807No Hit
TTTCTAAATTTTCCACCTTTTTCAG21950.11962583002341287No Hit
GAATATGGCAAGAAAACTGAAAATC21550.11744586045578803No Hit
GAGTACTTTTTTTTTTTTTTTTTTT19780.10779949511904813No Hit
ATTCCAGGTCCTTCAGTGTGCATTT18610.10142308413374548No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACAACG456.771764E-414.7752977
GGACCGT602.5728286E-514.2476076
GGTATCA16950.013.9797031
CCAACGT707.299426E-613.56508319
TAGGACC8800.013.3954354
TGGACCG751.467837E-512.6759425
CGGTTTC853.9614606E-612.28937413
AATCCCG1501.4551915E-1112.02770719
TGGTATC5050.011.671272
GTGGTAT5550.011.65961551
GGACCTG18750.011.550066
AGGACCT19200.011.14096455
AGACCGT600.005883691411.0814726
TAGAAAT8850.010.95657354
GCCGGTT1053.4736986E-610.8553211
CACCTTT7900.010.81801914
TCCAACG1502.1500455E-910.76163318
CCACCTT8000.010.56467213
GACCGTG909.53251E-510.5537837
GTCCTAA17500.010.5495031
Produced by FastQC (version 0.11.5)