##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512433_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1834888 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.818089714467586 33.0 31.0 34.0 30.0 34.0 2 31.987303857238153 34.0 31.0 34.0 30.0 34.0 3 32.11194307227471 34.0 31.0 34.0 30.0 34.0 4 35.55701982900319 37.0 35.0 37.0 33.0 37.0 5 35.35252505875018 37.0 35.0 37.0 33.0 37.0 6 35.38789942492403 37.0 35.0 37.0 33.0 37.0 7 35.29346586821648 37.0 35.0 37.0 33.0 37.0 8 35.256216728214476 37.0 35.0 37.0 33.0 37.0 9 36.88678164552823 39.0 37.0 39.0 33.0 39.0 10 36.82019556506991 39.0 37.0 39.0 33.0 39.0 11 36.854027057782275 39.0 37.0 39.0 33.0 39.0 12 36.77544296981614 39.0 37.0 39.0 33.0 39.0 13 36.75234128731563 39.0 37.0 39.0 33.0 39.0 14 38.05766455500281 40.0 38.0 41.0 33.0 41.0 15 38.052930206094324 40.0 38.0 41.0 33.0 41.0 16 38.01233644778319 40.0 38.0 41.0 33.0 41.0 17 38.0157159456054 40.0 38.0 41.0 33.0 41.0 18 37.96925207424104 40.0 38.0 41.0 33.0 41.0 19 37.99135478568719 40.0 38.0 41.0 33.0 41.0 20 37.976643261060076 40.0 38.0 41.0 33.0 41.0 21 37.92243232284477 40.0 38.0 41.0 33.0 41.0 22 37.868666098421265 40.0 38.0 41.0 33.0 41.0 23 37.78743062246851 40.0 38.0 41.0 33.0 41.0 24 37.73534678955882 40.0 38.0 41.0 33.0 41.0 25 37.661377697167346 40.0 38.0 41.0 33.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.43179142452431307 1101 2 0.5217082631676959 1101 3 0.3941743795734496 1101 4 0.38866940611749357 1101 5 0.3979611150038238 1101 6 0.4601861119563182 1101 7 0.4770037273866734 1101 8 0.584141740933795 1101 9 0.541713291864248 1101 10 0.5418746616724732 1101 11 0.5203437717166395 1101 12 0.5613868743730706 1101 13 0.701835065124456 1101 14 0.7590318865463033 1101 15 0.7501941646634336 1101 16 0.7073112179269927 1101 17 0.8031724472513915 1101 18 0.6659211029479408 1101 19 0.5769428311488696 1101 20 0.74834108983557 1101 21 0.741308082756305 1101 22 0.6354546145369042 1101 23 0.8089160979677317 1101 24 0.8482113287832576 1101 25 0.7026483222341966 1102 1 0.5675888913408187 1102 2 0.5256769173000606 1102 3 0.5358941499467491 1102 4 0.40759295500429005 1102 5 0.3455457822577159 1102 6 0.5203279580821274 1102 7 0.5450789331698687 1102 8 0.4316617117748507 1102 9 0.6173874406356248 1102 10 0.5963964245737898 1102 11 0.5956300457499921 1102 12 0.6655362532267617 1102 13 0.6884423001880862 1102 14 0.731502499795404 1102 15 0.7100747952256583 1102 16 0.8215732839598573 1102 17 0.8675894201882457 1102 18 0.7577377660709885 1102 19 0.7845487601346832 1102 20 0.8401182668850495 1102 21 0.8293459286391283 1102 22 0.8355238670351639 1102 23 0.8620136285694358 1102 24 0.7695347197257618 1102 25 0.7963478635882666 1103 1 0.4208599974583933 1103 2 0.43082132189220346 1103 3 0.41309133243670004 1103 4 0.36474663606099966 1103 5 0.30819389599523817 1103 6 0.32657660766511754 1103 7 0.4258858703520687 1103 8 0.4173235241425104 1103 9 0.4879934357669242 1103 10 0.44794389443247695 1103 11 0.4892474347997293 1103 12 0.571379273551436 1103 13 0.5726403325404803 1103 14 0.6438240646946412 1103 15 0.7522884708086721 1103 16 0.663742305753793 1103 17 0.6559333407909307 1103 18 0.6841327648466091 1103 19 0.6152063231901579 1103 20 0.6994288699992879 1103 21 0.658477196374605 1103 22 0.6315586105639639 1103 23 0.6518256505218147 1103 24 0.6992124922219034 1103 25 0.7468164402847464 1104 1 0.277790426161161 1104 2 0.26131774065228086 1104 3 0.24634814119683313 1104 4 0.0565766744333942 1104 5 0.07592805293117522 1104 6 0.0517702588782285 1104 7 0.059929395767582605 1104 8 0.1546911889318494 1104 9 0.15901980095980406 1104 10 0.16549059369090457 1104 11 0.1415885912566992 1104 12 0.21007042808152931 1104 13 0.1438736994565346 1104 14 0.12339090257815855 1104 15 0.18791313704592483 1104 16 0.26901644540287606 1104 17 0.40784617985259786 1104 18 0.396884291209048 1104 19 0.43965454798522074 1104 20 0.43665559744518134 1104 21 0.4201642604185096 1104 22 0.3930810641950231 1104 23 0.2999337318470694 1104 24 0.31911658876398263 1104 25 0.49143356967734064 1105 1 0.30923968764330567 1105 2 0.37596731712186937 1105 3 0.34807312310633876 1105 4 0.21955447633182956 1105 5 0.24039459615681125 1105 6 0.2582275014611213 1105 7 0.2865011405426259 1105 8 0.2377356064179068 1105 9 0.2809039792256698 1105 10 0.3303077732763171 1105 11 0.3246213031283176 1105 12 0.37698184850463434 1105 13 0.4002367650505434 1105 14 0.50829278824623 1105 15 0.4975149333256681 1105 16 0.39245534209550925 1105 17 0.4530708212351371 1105 18 0.39014504973286535 1105 19 0.3912443832552981 1105 20 0.3220661452679394 1105 21 0.43606048482578075 1105 22 0.38051189465516444 1105 23 0.5380365709906414 1105 24 0.4355635091232415 1105 25 0.4845219364248834 1106 1 0.4356188978639821 1106 2 0.4421725638016625 1106 3 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0.6972229894971349 1107 16 0.695410679441089 1107 17 0.8362924979324191 1107 18 0.7577804630999836 1107 19 0.7631290839204894 1107 20 0.7747775801744936 1107 21 0.8289260745206448 1107 22 0.7754989604349092 1107 23 0.7028248721204093 1107 24 0.6075493078773349 1107 25 0.6674028360130961 1108 1 0.36404407628915436 1108 2 0.3618413008617054 1108 3 0.4025401733050984 1108 4 0.3335786168803594 1108 5 0.4445273042529365 1108 6 0.30114314437676626 1108 7 0.3289004134297002 1108 8 0.21508199382527238 1108 9 0.427294882323892 1108 10 0.3293559345195973 1108 11 0.2664724946746304 1108 12 0.3013371350550429 1108 13 0.38052805967110714 1108 14 0.5395466585244932 1108 15 0.5303433826175308 1108 16 0.6566040806344944 1108 17 0.7098902515301759 1108 18 0.5564466617661807 1108 19 0.47507011586152004 1108 20 0.6542019395988561 1108 21 0.5542612798558437 1108 22 0.5260640710000217 1108 23 0.6398413691369029 1108 24 0.6583179986460266 1108 25 0.6284558370660989 1109 1 -0.07021271014154351 1109 2 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-0.9329456627839363 2315 8 -0.8180989093424103 2315 9 -1.088562799950715 2315 10 -1.0108645135016445 2315 11 -1.0431137131927528 2315 12 -1.1819074942451167 2315 13 -1.1779406939496937 2315 14 -1.2039080666843134 2315 15 -1.235436171526942 2315 16 -1.4203799028281168 2315 17 -1.3926374614867427 2315 18 -1.3850366140493975 2315 19 -1.2985512718629977 2315 20 -1.1848913626059456 2315 21 -1.2474405381527163 2315 22 -1.208051662586847 2315 23 -1.2175924980810109 2315 24 -1.294938314680806 2315 25 -1.1612780291772395 2316 1 -1.3049858961916776 2316 2 -1.3941226901360437 2316 3 -1.1344674815570208 2316 4 -1.0880822651706197 2316 5 -1.0081793613569516 2316 6 -1.0626027060907646 2316 7 -1.0710348898064268 2316 8 -1.0438359297588207 2316 9 -1.3660710267209382 2316 10 -1.3068926853749332 2316 11 -1.4151829314669158 2316 12 -1.331486427100856 2316 13 -1.4112972922442708 2316 14 -1.560456106294481 2316 15 -1.6484552482441188 2316 16 -1.7961116167173614 2316 17 -1.8843286963271808 2316 18 -2.019189753480134 2316 19 -2.0796629777312887 2316 20 -1.9442916027056398 2316 21 -1.8979635699684039 2316 22 -2.0004732144269894 2316 23 -2.1151831363752223 2316 24 -2.107386633900319 2316 25 -2.051425312582488 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 14803.0 3 0.0 4 320.0 5 2433.0 6 3159.0 7 2437.0 8 1977.0 9 1762.0 10 1604.0 11 1583.0 12 1487.0 13 1435.0 14 1504.0 15 1461.0 16 1522.0 17 1614.0 18 1814.0 19 2139.0 20 2575.0 21 3329.0 22 4255.0 23 5260.0 24 6493.0 25 7939.0 26 8820.0 27 10377.0 28 11900.0 29 14477.0 30 18555.0 31 24607.0 32 32065.0 33 42935.0 34 57903.0 35 79584.0 36 119571.0 37 227105.0 38 1114084.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.98911374342127 19.761487151520512 13.692925898449165 24.556473206609052 2 14.478567196848017 20.2881978830171 42.40451585230063 22.828719067834253 3 18.833411085581243 24.965392983113958 30.264572006574785 25.93662392473001 4 12.478268215186906 17.248306419528358 38.265653697537154 32.00777166774758 5 12.575486090044238 38.4962690720367 35.9045345124477 13.023710325471358 6 31.56328706782821 37.46006738118342 17.926393144128134 13.050252406860233 7 27.707626490366582 31.81660096116107 22.252361473315133 18.223411075157216 8 24.476651265826924 38.40156508580935 20.40525867087628 16.716524977487445 9 26.23353357724601 14.800113178971607 20.871052842476786 38.09530040130559 10 15.798947954322312 28.716736510477116 34.456322223197446 21.027993312003122 11 33.71705244839691 21.881748363492036 25.947735330308287 18.453463857802767 12 24.266448017602414 24.317911959587722 31.98522156208329 19.430418460726578 13 29.47905730336956 22.355029921252974 25.991097214363514 22.174815561013954 14 20.146372410304934 23.6793999472414 27.820350430462206 28.353877211991463 15 22.144984725397805 33.38643789736449 23.341009241066196 21.12756813617151 16 20.836523867008562 28.171762891345637 29.633894776426473 21.35781846521932 17 20.573672705393655 29.725611334589363 28.702437599427537 20.998278360589445 18 20.561224406378397 26.65944003998079 32.47153357629187 20.30780197734894 19 23.3584829155785 26.40896883079512 28.56975466622486 21.66279358740152 20 23.670709056901565 27.059308252056802 29.460435732317176 19.809546958724454 21 22.0894136317857 26.53954900789585 28.130490798348458 23.240546561969992 22 23.05596083716981 28.994915524898886 28.05699364672245 19.892129991208858 23 21.08159102193015 28.641359528817013 29.449481734683257 20.827567714569582 24 23.239674574142946 26.391801570450074 30.013276014666836 20.35524784074014 25 21.794899743199586 27.91859775637532 29.720070107821293 20.5664323926038 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 218.0 1 218.0 2 466.5 3 715.0 4 715.0 5 715.0 6 1887.5 7 3060.0 8 3060.0 9 3060.0 10 3913.0 11 4766.0 12 4766.0 13 4766.0 14 6485.0 15 8204.0 16 8204.0 17 8204.0 18 15108.0 19 22012.0 20 22012.0 21 22012.0 22 36148.5 23 50285.0 24 50285.0 25 50285.0 26 75845.5 27 101406.0 28 101406.0 29 101406.0 30 130484.5 31 159563.0 32 159563.0 33 159563.0 34 175691.5 35 191820.0 36 191820.0 37 191820.0 38 200335.5 39 208851.0 40 208851.0 41 208851.0 42 217244.0 43 225637.0 44 225637.0 45 225637.0 46 233356.0 47 241075.0 48 241075.0 49 241075.0 50 227824.5 51 214574.0 52 214574.0 53 214574.0 54 186280.0 55 157986.0 56 157986.0 57 157986.0 58 134385.0 59 110784.0 60 110784.0 61 110784.0 62 91188.0 63 71592.0 64 71592.0 65 71592.0 66 53948.0 67 36304.0 68 36304.0 69 36304.0 70 26584.5 71 16865.0 72 16865.0 73 16865.0 74 11601.5 75 6338.0 76 6338.0 77 6338.0 78 4215.0 79 2092.0 80 2092.0 81 2092.0 82 1316.5 83 541.0 84 541.0 85 541.0 86 347.5 87 154.0 88 154.0 89 154.0 90 95.0 91 36.0 92 36.0 93 36.0 94 22.0 95 8.0 96 8.0 97 8.0 98 5.0 99 2.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.16551418942191568 2 0.005831418593396436 3 0.0 4 9.809863054311763E-4 5 0.11444840230030388 6 0.02986558307646025 7 0.023979665243873197 8 0.01940172915186104 9 0.03406202449413806 10 0.03035607622917584 11 0.03526100775633172 12 0.03237254807922882 13 0.022126691111392083 14 0.006321911746112023 15 0.007793391204258788 16 0.0017984748932904895 17 4.904931527155882E-4 18 3.814946743343463E-4 19 0.0 20 0.0 21 0.0 22 0.004741433809584018 23 9.264870662405553E-4 24 0.0 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1834888.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.39647402578805 #Duplication Level Percentage of deduplicated Percentage of total 1 71.9856613277615 38.43780495316992 2 16.228358573473407 17.33074254099295 3 5.671341986298044 9.084889951881742 4 2.4866248243343403 5.311079913777935 5 1.2579416318488783 3.3584823835488034 6 0.6995662455777616 2.241262251678661 7 0.40423035163470905 1.510913283004854 8 0.2774131711115612 1.185030814853598 9 0.19273536771085023 0.926225015024081 >10 0.6963045205496833 6.313456210364694 >50 0.048123731233433234 1.819718526938453 >100 0.0418908522598497 4.579459458160762 >500 0.00541451103035216 2.0012813710096156 >1k 0.0036777810772203354 3.2737727314790273 >5k 7.151240983483986E-4 2.6258805941148053 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 9998 0.5448833934278278 No Hit TATCAACGCAGAGTACTTTTTTTTT 6630 0.36132995583381655 No Hit GGTATCAACGCAGAGTACTTTTTTT 6623 0.3609484611594822 No Hit CTGTAGGACGTGGAATATGGCAAGA 6604 0.3599129756148604 No Hit GTCCTACAGTGGACATTTCTAAATT 6528 0.35577103343637323 No Hit GTCCTAAAGTGTGTATTTCTCATTT 6072 0.3309193803654501 No Hit CTTTAGGACGTGAAATATGGCGAGG 5682 0.30966467708110795 No Hit GTCCTACAGTGTGCATTTCTCATTT 3364 0.18333544063724871 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 3141 0.17118211029774025 No Hit CTGAAGGACCTGGAATATGGCGAGA 2821 0.15374235375674156 No Hit CTGTAGGACCTGGAATATGGCGAGA 2643 0.14404148918081103 No Hit ATTTAGAAATGTCCACTGTAGGACG 2549 0.13891856069689268 No Hit GTACTGGTTCACTATCGGTCAGTCA 2370 0.12916319688177155 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 2304 0.12556624709519057 No Hit GTCCTTCAGTGTGCATTTCTCATTT 2300 0.12534825013842807 No Hit TTTCTAAATTTTCCACCTTTTTCAG 2195 0.11962583002341287 No Hit GAATATGGCAAGAAAACTGAAAATC 2155 0.11744586045578803 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 1978 0.10779949511904813 No Hit ATTCCAGGTCCTTCAGTGTGCATTT 1861 0.10142308413374548 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 5.44992391906209E-5 0.0 10 0.0 0.0 0.0 5.44992391906209E-5 0.0 11 0.0 0.0 0.0 5.44992391906209E-5 0.0 12 0.0 0.0 0.0 5.44992391906209E-5 0.0 13 0.0 0.0 0.0 5.44992391906209E-5 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GACAACG 45 6.771764E-4 14.775297 7 GGACCGT 60 2.5728286E-5 14.247607 6 GGTATCA 1695 0.0 13.979703 1 CCAACGT 70 7.299426E-6 13.565083 19 TAGGACC 880 0.0 13.395435 4 TGGACCG 75 1.467837E-5 12.675942 5 CGGTTTC 85 3.9614606E-6 12.289374 13 AATCCCG 150 1.4551915E-11 12.027707 19 TGGTATC 505 0.0 11.67127 2 GTGGTAT 555 0.0 11.6596155 1 GGACCTG 1875 0.0 11.55006 6 AGGACCT 1920 0.0 11.1409645 5 AGACCGT 60 0.0058836914 11.081472 6 TAGAAAT 885 0.0 10.9565735 4 GCCGGTT 105 3.4736986E-6 10.85532 11 CACCTTT 790 0.0 10.818019 14 TCCAACG 150 2.1500455E-9 10.761633 18 CCACCTT 800 0.0 10.564672 13 GACCGTG 90 9.53251E-5 10.553783 7 GTCCTAA 1750 0.0 10.549503 1 >>END_MODULE