FastQCFastQC Report
Thu 26 May 2016
SRR1512432_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512432_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1916524
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT93160.4860883557941356No Hit
GTCCTACAGTGGACATTTCTAAATT59230.30904909095842265No Hit
CTGTAGGACGTGGAATATGGCAAGA56990.29736126445585864No Hit
GTCCTAAAGTGTGTATTTCTCATTT55510.28963895051666455No Hit
GGTATCAACGCAGAGTACTTTTTTT55250.288282327797617No Hit
TATCAACGCAGAGTACTTTTTTTTT53480.2790468577487159No Hit
CTTTAGGACGTGAAATATGGCGAGG46720.24377466705347806No Hit
GTCCTACAGTGTGCATTTCTCATTT31540.16456877137985226No Hit
GTACTGGTTCACTATCGGTCAGTCA29430.15355925623681208No Hit
ACGCAGAGTACTTTTTTTTTTTTTT26060.1359753386860796No Hit
CTGAAGGACCTGGAATATGGCGAGA24410.12736600219981592No Hit
GTACTTTTTTTTTTTTTTTTTTTTT22410.11693044282252661No Hit
GTCCTTCAGTGTGCATTTCTCATTT21850.11400848619688561No Hit
CTGTAGGACCTGGAATATGGCGAGA20290.10586874988259996No Hit
GAATATGGCAAGAAAACTGAAAATC19620.10237283749120804No Hit
ATTTAGAAATGTCCACTGTAGGACG19250.10044225900640953No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14950.014.7171781
GGACCGT653.4317673E-614.5911136
TAGGACC9650.012.3832224
GTCCTAG2400.011.9219591
TAGAAAT7650.011.9014594
TCCAACG1603.6379788E-1211.842854518
AATCCCG2650.011.80063719
ATAATAC1501.8189894E-1011.3810683
ACGAAAT2250.011.37748712
TTTAGAA8700.011.2284492
GTATCAA34750.011.1299231
GTCTTAC2050.011.06967451
GGGACCG600.005949629511.0649275
GAAATGT8150.010.9388596
CGAAATC2100.010.84081813
ATGTCCA7450.010.79233559
GGCGAGG14350.010.76398619
TGTAGGA34300.010.728512
ATTTAGA7450.010.727281
AAATGTC7700.010.715947
Produced by FastQC (version 0.11.5)