FastQCFastQC Report
Thu 26 May 2016
SRR1512432_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512432_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1916524
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT116590.6083409338990797No Hit
GGTATCAACGCAGAGTACTTTTTTT78380.4089695719959677No Hit
TATCAACGCAGAGTACTTTTTTTTT76610.3997341019470667No Hit
CTGTAGGACGTGGAATATGGCAAGA66150.34515612640384363No Hit
GTCCTACAGTGGACATTTCTAAATT61200.31932811694505264No Hit
CTTTAGGACGTGAAATATGGCGAGG55880.29156952900146305No Hit
GTCCTAAAGTGTGTATTTCTCATTT55460.2893780615322323No Hit
ACGCAGAGTACTTTTTTTTTTTTTT37460.19545802713662863No Hit
GTCCTACAGTGTGCATTTCTCATTT32950.17192584074084125No Hit
CTGAAGGACCTGGAATATGGCGAGA29390.1533505450492663No Hit
GTACTGGTTCACTATCGGTCAGTCA28830.15042858842362528No Hit
GTACTTTTTTTTTTTTTTTTTTTTT26500.13827116174908324No Hit
CTGTAGGACCTGGAATATGGCGAGA24340.12700075762161078No Hit
GAGTACTTTTTTTTTTTTTTTTTTT23900.12470493455860715No Hit
ATTTAGAAATGTCCACTGTAGGACG23200.12105248877655589No Hit
TTTCTAAATTTTCCACCTTTTTCAG22190.1157825312910248No Hit
GAATATGGCAAGAAAACTGAAAATC21880.11416501958754495No Hit
GTCCTTCAGTGTGCATTTCTCATTT21530.11233879669651932No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTACG704.4045737E-714.9384024
GGTATCA19950.014.7324411
TAGGACC9950.012.5157744
AGTACGT853.9111637E-612.30355
GGCGAGG16100.012.14868619
CGTATAC550.003053798312.0988723
AAACGGG550.003081388512.08435219
CCGGTTT951.043396E-611.99693412
GGCGAAA951.0470194E-611.9934919
CTACACG802.8479228E-511.882824
CGCTACT657.9842185E-411.6993962
TCCAACG1800.011.60481318
GCCGGTT1157.088602E-811.56377211
GTCTTAC1657.2759576E-1211.5293611
CTGTTCG752.0736862E-411.400369
ACCGTGC752.0736862E-411.400368
GGACCGT1001.9294257E-611.400366
TAGAAAT7950.011.2401014
CCAACGA1105.0048584E-711.22118419
AAATGTC7250.011.1382837
Produced by FastQC (version 0.11.5)