##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512432_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1916524 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.632548822764548 33.0 31.0 34.0 30.0 34.0 2 31.80705642089533 34.0 31.0 34.0 30.0 34.0 3 31.924551949258134 34.0 31.0 34.0 30.0 34.0 4 35.39762611895286 37.0 35.0 37.0 33.0 37.0 5 35.18408900697304 37.0 35.0 37.0 33.0 37.0 6 35.21392374945474 37.0 35.0 37.0 33.0 37.0 7 35.1108929499448 37.0 35.0 37.0 32.0 37.0 8 35.070927366419625 37.0 35.0 37.0 32.0 37.0 9 36.667397329749065 39.0 37.0 39.0 33.0 39.0 10 36.5835601328238 39.0 37.0 39.0 32.0 39.0 11 36.62270443782598 39.0 37.0 39.0 33.0 39.0 12 36.54497673913815 39.0 37.0 39.0 32.0 39.0 13 36.51802586349036 39.0 37.0 39.0 32.0 39.0 14 37.777350035793965 40.0 38.0 41.0 32.0 41.0 15 37.76046999672324 40.0 38.0 41.0 32.0 41.0 16 37.71286454017795 40.0 38.0 41.0 32.0 41.0 17 37.716724653591605 40.0 38.0 41.0 32.0 41.0 18 37.67422427269369 40.0 38.0 41.0 32.0 41.0 19 37.69176905689676 40.0 38.0 41.0 32.0 41.0 20 37.679652328903785 40.0 38.0 41.0 32.0 41.0 21 37.61940523572885 40.0 38.0 41.0 32.0 41.0 22 37.55804362481242 40.0 38.0 41.0 32.0 41.0 23 37.468931774399906 40.0 38.0 41.0 32.0 41.0 24 37.41214354738057 40.0 38.0 41.0 32.0 41.0 25 37.33822743675529 40.0 38.0 41.0 32.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.5139511493929803 1101 2 0.4177033679040889 1101 3 0.36706724984731665 1101 4 0.35163452969784714 1101 5 0.369798594078361 1101 6 0.5075890946561188 1101 7 0.48735904773857186 1101 8 0.4522636880457753 1101 9 0.6594551380960283 1101 10 0.5182405180268645 1101 11 0.5512603492834955 1101 12 0.5723044055492892 1101 13 0.5367762196119372 1101 14 0.6423823168549845 1101 15 0.6425785819997358 1101 16 0.5789188780769123 1101 17 0.6859271447147393 1101 18 0.6105596225478749 1101 19 0.5725284022448207 1101 20 0.6453729510742221 1101 21 0.5035288998680585 1101 22 0.50988515513734 1101 23 0.5880199480526969 1101 24 0.6423862393460453 1101 25 0.6377759317259901 1102 1 0.60742153920026 1102 2 0.6399308483304225 1102 3 0.617073545854872 1102 4 0.4468063636570463 1102 5 0.4593748686186032 1102 6 0.5317425168752408 1102 7 0.6644567460012283 1102 8 0.6377449008976015 1102 9 0.7124124489508148 1102 10 0.787295073555029 1102 11 0.7244215898986397 1102 12 0.8302389663688672 1102 13 0.6734081627591237 1102 14 0.9599745243071922 1102 15 0.973395172796053 1102 16 1.01015858825577 1102 17 0.991535351498321 1102 18 0.9220036056540906 1102 19 0.9850275962174777 1102 20 0.9787361731467215 1102 21 1.0037354919401835 1102 22 0.9407235813181032 1102 23 0.9502991415327458 1102 24 0.8653650894653637 1102 25 0.9126884033835765 1103 1 0.38825590199053295 1103 2 0.470537063811598 1103 3 0.39139009083771015 1103 4 0.373576314637873 1103 5 0.39477804641665415 1103 6 0.4166977427355505 1103 7 0.40084304421442596 1103 8 0.43274918803529516 1103 9 0.4048533488233801 1103 10 0.4977756382676475 1103 11 0.47377483478025795 1103 12 0.5286460670689621 1103 13 0.5405374227238937 1103 14 0.6654255317187534 1103 15 0.714145456105058 1103 16 0.6618550999626294 1103 17 0.6993386263556971 1103 18 0.6645224319521148 1103 19 0.7411186740760058 1103 20 0.6962185364043947 1103 21 0.7634884012197176 1103 22 0.7047428373825966 1103 23 0.7468422189765036 1103 24 0.7091861634446701 1103 25 0.7342542587136052 1104 1 0.46408661008828034 1104 2 0.4689507758393958 1104 3 0.4543031040962404 1104 4 0.37725940580880746 1104 5 0.33614062914315923 1104 6 0.45251555047764924 1104 7 0.39809825080093475 1104 8 0.4112238250146305 1104 9 0.6452091687712738 1104 10 0.5644998049834413 1104 11 0.6245435305117013 1104 12 0.554160929477554 1104 13 0.5352474842581927 1104 14 0.6626559680371216 1104 15 0.6670737724352591 1104 16 0.7419007168884519 1104 17 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-1.1063991746194546 2314 8 -1.1407993230907252 2314 9 -1.4219918271642484 2314 10 -1.258213194871729 2314 11 -1.27450706436872 2314 12 -1.159969830200616 2314 13 -1.3618278795879917 2314 14 -1.5440037344764477 2314 15 -1.6111133290903865 2314 16 -1.711447161047822 2314 17 -1.921406600652766 2314 18 -1.822328574987793 2314 19 -1.5152989199419835 2314 20 -1.5495631952025448 2314 21 -1.4714975081275625 2314 22 -1.4675077487699681 2314 23 -1.6736572470902402 2314 24 -1.573873061322395 2314 25 -1.5916159937930487 2315 1 -1.933589459731813 2315 2 -1.5185086003286479 2315 3 -0.9998442528450084 2315 4 -0.6839043062463759 2315 5 -0.7052480301267536 2315 6 -0.6435202589174196 2315 7 -0.7521691684459171 2315 8 -0.6248344701974773 2315 9 -0.806746425238245 2315 10 -0.8064334921102585 2315 11 -0.670219750399248 2315 12 -0.8103039320341097 2315 13 -0.7786490941280775 2315 14 -1.0722359098678211 2315 15 -1.0035223646090756 2315 16 -0.9983535386361666 2315 17 -1.026626435451405 2315 18 -0.9742297179407586 2315 19 -0.8006140772312165 2315 20 -0.7795727182404875 2315 21 -0.9324766037090129 2315 22 -0.7860314915729987 2315 23 -0.8255079382484283 2315 24 -0.9808588973473604 2315 25 -0.8735184325825642 2316 1 -1.3159019261066653 2316 2 -1.3355637243837748 2316 3 -1.1223744496716925 2316 4 -0.9010319129576629 2316 5 -1.0942503760224653 2316 6 -1.0877840495613142 2316 7 -1.101737643048736 2316 8 -1.1252384534693292 2316 9 -1.4624777232363328 2316 10 -1.2676844824069562 2316 11 -1.4540360553374398 2316 12 -1.5279254030789247 2316 13 -1.546281832405981 2316 14 -1.639821653190623 2316 15 -1.7545243779448612 2316 16 -1.7748889960729883 2316 17 -2.0152060835542045 2316 18 -2.054672465630489 2316 19 -2.0615598250223854 2316 20 -1.9568761130481889 2316 21 -2.3311711775893187 2316 22 -2.1962804364335327 2316 23 -2.271311102575403 2316 24 -2.07553816319885 2316 25 -1.9631876317551473 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 18430.0 3 0.0 4 406.0 5 3096.0 6 3855.0 7 2986.0 8 2458.0 9 2186.0 10 1944.0 11 1804.0 12 1755.0 13 1760.0 14 1755.0 15 1715.0 16 1818.0 17 1921.0 18 2136.0 19 2387.0 20 2959.0 21 3894.0 22 4993.0 23 6229.0 24 7502.0 25 9009.0 26 10140.0 27 11580.0 28 13439.0 29 16410.0 30 21247.0 31 27423.0 32 35519.0 33 47681.0 34 63536.0 35 87814.0 36 131440.0 37 259513.0 38 1103784.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.251912146809396 19.71251429276175 13.81307889604485 24.222494664384005 2 14.700681212772674 20.427773443920614 42.38369681204945 22.487848531257256 3 19.032425370097116 25.369105735174724 30.22513675800564 25.373332136722524 4 12.573577714920498 17.46037460781597 38.28597905255957 31.680068624703956 5 12.505889173844235 38.71284336103378 35.75143768966796 13.029829775454028 6 31.279524384381848 37.654729825297586 17.986890403501874 13.078855386818692 7 27.671932212447757 31.986624943113735 22.13914276051822 18.20230008392029 8 24.57737463710294 38.25387738189113 20.2687956279613 16.899952353044633 9 26.33921324956667 14.909572605230704 20.666278001019855 38.08493614418278 10 15.693559801840056 28.76226234689681 34.29553221560436 21.248645635658768 11 33.679587793303725 22.001198372384824 25.875396412816322 18.443817421495126 12 24.388726513569935 24.424791231732776 32.033716075156576 19.15276617954071 13 29.56148211082692 22.369751153218495 26.008488857135553 22.060277878819036 14 20.299415570862035 23.637862659152578 27.85791066583177 28.20481110415362 15 22.22873340214427 32.966475018811686 23.629930044532298 21.174861534511745 16 20.924134944260224 28.201430215054046 29.390788914137527 21.483645926548203 17 20.687819948562993 29.538345302081538 28.603108030757845 21.170726718597624 18 20.620468996377806 26.571365361556744 32.42521072069242 20.38295492137303 19 23.279176258684995 26.41819251937362 28.705562779281657 21.59706844265973 20 23.628350075449095 26.943883823004565 29.390135474431833 20.037630627114506 21 22.33157528943024 26.42591483331281 28.24488501057122 22.99762486668573 22 23.19048617916214 28.631368141369425 28.25035195209486 19.927793727373576 23 21.329369893405588 28.265076699032672 29.50576488494493 20.89978852261681 24 23.173568397786827 26.42815847857893 29.94499416652231 20.45327895711194 25 21.896882063569254 27.775128305202546 29.742961736977986 20.585027894250217 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 292.0 1 292.0 2 578.5 3 865.0 4 865.0 5 865.0 6 2177.5 7 3490.0 8 3490.0 9 3490.0 10 4491.0 11 5492.0 12 5492.0 13 5492.0 14 7380.0 15 9268.0 16 9268.0 17 9268.0 18 16784.5 19 24301.0 20 24301.0 21 24301.0 22 39158.0 23 54015.0 24 54015.0 25 54015.0 26 80310.0 27 106605.0 28 106605.0 29 106605.0 30 137283.0 31 167961.0 32 167961.0 33 167961.0 34 183377.0 35 198793.0 36 198793.0 37 198793.0 38 205942.0 39 213091.0 40 213091.0 41 213091.0 42 221957.0 43 230823.0 44 230823.0 45 230823.0 46 240700.0 47 250577.0 48 250577.0 49 250577.0 50 236801.0 51 223025.0 52 223025.0 53 223025.0 54 194096.0 55 165167.0 56 165167.0 57 165167.0 58 141026.0 59 116885.0 60 116885.0 61 116885.0 62 96980.5 63 77076.0 64 77076.0 65 77076.0 66 58443.5 67 39811.0 68 39811.0 69 39811.0 70 29238.5 71 18666.0 72 18666.0 73 18666.0 74 12884.5 75 7103.0 76 7103.0 77 7103.0 78 4690.0 79 2277.0 80 2277.0 81 2277.0 82 1483.5 83 690.0 84 690.0 85 690.0 86 444.0 87 198.0 88 198.0 89 198.0 90 119.0 91 40.0 92 40.0 93 40.0 94 24.5 95 9.0 96 9.0 97 9.0 98 6.5 99 4.0 100 4.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.15486370115897322 2 0.004643823922893739 3 0.0 4 6.783113595238045E-4 5 0.10404252699157433 6 0.027288987771611525 7 0.021914674692307533 8 0.01810569551959694 9 0.029584810834615168 10 0.025880187255677468 11 0.03088925575677633 12 0.027341165568497965 13 0.019044895863552975 14 0.006470046813919367 15 0.008452803095604334 16 0.0015131561097069485 17 2.6088898443223254E-4 18 3.13066781318679E-4 19 0.0 20 0.0 21 0.0 22 0.003652445782051255 23 3.6524457820512557E-4 24 0.0 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1916524.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.78128011377836 #Duplication Level Percentage of deduplicated Percentage of total 1 77.92644447231531 45.806161607979604 2 14.000024727718541 16.45878750239694 3 4.124248325425406 7.272857882268363 4 1.5804109411266587 3.7159431290098475 5 0.768750596216667 2.259407206692301 6 0.39911185648205283 1.407618349956099 7 0.247371533593014 1.0178570785814123 8 0.1700925911471383 0.799860819639865 9 0.1153196402001991 0.6100772465905233 >10 0.5757045415928111 5.999712086745447 >50 0.04313913597428643 1.7728927783052202 >100 0.040517539325397006 4.832772871529115 >500 0.004609331418946792 1.8855506956377321 >1k 0.0036342805418618923 3.5017742235471707 >5k 5.318459329553989E-4 2.0512423948785843 >10k+ 8.864098882589982E-5 0.6074841262418567 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 11659 0.6083409338990797 No Hit GGTATCAACGCAGAGTACTTTTTTT 7838 0.4089695719959677 No Hit TATCAACGCAGAGTACTTTTTTTTT 7661 0.3997341019470667 No Hit CTGTAGGACGTGGAATATGGCAAGA 6615 0.34515612640384363 No Hit GTCCTACAGTGGACATTTCTAAATT 6120 0.31932811694505264 No Hit CTTTAGGACGTGAAATATGGCGAGG 5588 0.29156952900146305 No Hit GTCCTAAAGTGTGTATTTCTCATTT 5546 0.2893780615322323 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 3746 0.19545802713662863 No Hit GTCCTACAGTGTGCATTTCTCATTT 3295 0.17192584074084125 No Hit CTGAAGGACCTGGAATATGGCGAGA 2939 0.1533505450492663 No Hit GTACTGGTTCACTATCGGTCAGTCA 2883 0.15042858842362528 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 2650 0.13827116174908324 No Hit CTGTAGGACCTGGAATATGGCGAGA 2434 0.12700075762161078 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 2390 0.12470493455860715 No Hit ATTTAGAAATGTCCACTGTAGGACG 2320 0.12105248877655589 No Hit TTTCTAAATTTTCCACCTTTTTCAG 2219 0.1157825312910248 No Hit GAATATGGCAAGAAAACTGAAAATC 2188 0.11416501958754495 No Hit GTCCTTCAGTGTGCATTTCTCATTT 2153 0.11233879669651932 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 5.2177796886446504E-5 0.0 0.0 5.2177796886446504E-5 0.0 11 5.2177796886446504E-5 0.0 0.0 5.2177796886446504E-5 0.0 12 5.2177796886446504E-5 0.0 0.0 5.2177796886446504E-5 0.0 13 5.2177796886446504E-5 0.0 0.0 5.2177796886446504E-5 5.2177796886446504E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGTACG 70 4.4045737E-7 14.938402 4 GGTATCA 1995 0.0 14.732441 1 TAGGACC 995 0.0 12.515774 4 AGTACGT 85 3.9111637E-6 12.3035 5 GGCGAGG 1610 0.0 12.148686 19 CGTATAC 55 0.0030537983 12.098872 3 AAACGGG 55 0.0030813885 12.084352 19 CCGGTTT 95 1.043396E-6 11.996934 12 GGCGAAA 95 1.0470194E-6 11.99349 19 CTACACG 80 2.8479228E-5 11.88282 4 CGCTACT 65 7.9842185E-4 11.699396 2 TCCAACG 180 0.0 11.604813 18 GCCGGTT 115 7.088602E-8 11.563772 11 GTCTTAC 165 7.2759576E-12 11.529361 1 CTGTTCG 75 2.0736862E-4 11.40036 9 ACCGTGC 75 2.0736862E-4 11.40036 8 GGACCGT 100 1.9294257E-6 11.40036 6 TAGAAAT 795 0.0 11.240101 4 CCAACGA 110 5.0048584E-7 11.221184 19 AAATGTC 725 0.0 11.138283 7 >>END_MODULE