FastQCFastQC Report
Thu 26 May 2016
SRR1512431_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512431_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2799312
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT88650.3166849568751179No Hit
CTGTAGGACGTGGAATATGGCAAGA77320.2762107260641186No Hit
GTCCTAAAGTGTGTATTTCTCATTT75190.2686017135639043No Hit
GTATCAACGCAGAGTACTTTTTTTT67640.24163080071103188No Hit
CTTTAGGACGTGAAATATGGCGAGG63760.2277702521190921No Hit
GTCCTACAGTGTGCATTTCTCATTT53110.18972518961802043No Hit
GGTATCAACGCAGAGTACTTTTTTT43060.15382351091982602No Hit
TATCAACGCAGAGTACTTTTTTTTT40650.14521425264493562No Hit
CTGTAGGACCTGGAATATGGCGAGA34830.12442342975702601No Hit
CTGAAGGACCTGGAATATGGCGAGA33700.12038672359494047No Hit
GTCCTTCAGTGTGCATTTCTCATTT30000.10716919014386393No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAACCG250.006093270618.967465
GGTATCA12200.013.7527281
TAGGACC15600.013.6784564
TACATCG701.10910085E-412.1933675
CCGTTTG550.003172017212.0379759
GGCGAGG18250.011.63090119
TGTAGGA51000.011.2503082
GGACCGC600.00595389111.0641546
GTCCTAC45000.010.8565651
CGACCTC700.001506059910.84497914
AATCCCG1752.1827873E-1110.82979319
AGGACCT30150.010.789125
GTAGGAC50000.010.7355823
CTGTAGG45150.010.6456211
GTCGTAC650.008022984510.6288061
GGACCTG29500.010.5765816
AAATGTC10750.010.41004857
CCACCTT10500.010.38899613
TGGCGAG45500.010.38889218
ATTTAGA10400.010.3441061
Produced by FastQC (version 0.11.5)