FastQCFastQC Report
Thu 26 May 2016
SRR1512431_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512431_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2799312
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGA93550.334189257931949No Hit
GTCCTACAGTGGACATTTCTAAATT93040.3323673816995033No Hit
GTATCAACGCAGAGTACTTTTTTTT83530.2983947484238984No Hit
GTCCTAAAGTGTGTATTTCTCATTT78340.27985447852900996No Hit
CTTTAGGACGTGAAATATGGCGAGG75890.2711023280005944No Hit
GTCCTACAGTGTGCATTTCTCATTT56850.20308561532262215No Hit
TATCAACGCAGAGTACTTTTTTTTT55170.19708414067456576No Hit
GGTATCAACGCAGAGTACTTTTTTT55090.19679835616751543No Hit
CTGTAGGACCTGGAATATGGCGAGA41340.14767914401824447No Hit
CTGAAGGACCTGGAATATGGCGAGA41000.1464645598632807No Hit
GTCCTTCAGTGTGCATTTCTCATTT31510.1125633727144384No Hit
ATTTAGAAATGTCCACTGTAGGACG30170.10777648222134581No Hit
GAATATGGCAAGAAAACTGAAAATC28410.10148922306623914No Hit
TTTCTAAATTTTCCACCTTTTTCAG28120.10045325422818178No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATCGC350.00217750316.27818115
GGGCGCG400.005287324514.2472267
GGTATCA16400.013.1125781
TAGGACC16250.013.1059494
TCCAACG1600.012.46253718
TCTTACG550.003051836312.100232
GGCGAGG19150.011.85048919
AGGACCT28350.011.603715
GGACCTG27200.011.4187336
GGCGCGC752.0787022E-411.397788
TGTCGAG1850.011.29166818
CCAACGA1201.285407E-711.07781119
TGTAGGA52050.010.90465452
CGGACAT700.001494696810.854835510
ATTTAGA11150.010.838081
TAGAAAT12300.010.7444334
CTGTAGG51550.010.724351
GTAGGAC52050.010.7041143
CTACACT5600.010.6961224
GTCGAGG1605.493348E-1010.68217519
Produced by FastQC (version 0.11.5)