FastQCFastQC Report
Thu 26 May 2016
SRR1512430_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512430_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2077244
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT63980.30800425948997806No Hit
GTCCTACAGTGGACATTTCTAAATT59780.2877851614928241No Hit
GTCCTAAAGTGTGTATTTCTCATTT54560.26265571112493286No Hit
CTGTAGGACGTGGAATATGGCAAGA52950.2549050568926905No Hit
CTTTAGGACGTGAAATATGGCGAGG48070.23141239064837835No Hit
GGTATCAACGCAGAGTACTTTTTTT43310.20849741291827056No Hit
TATCAACGCAGAGTACTTTTTTTTT37550.18076836423645948No Hit
GTCCTACAGTGTGCATTTCTCATTT26960.12978735285792137No Hit
CTGAAGGACCTGGAATATGGCGAGA25810.12425117126346255No Hit
GTCCTTCAGTGTGCATTTCTCATTT22970.11057920976062514No Hit
ATTTAGAAATGTCCACTGTAGGACG21850.10518745029471743No Hit
CTGTAGGACCTGGAATATGGCGAGA20940.1008066457286674No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTGCG457.009303E-414.7083999
GGTATCA13450.013.5730921
TAGGACC7750.012.7281744
GTGTAGG3900.012.3982641
TGGCGAA1157.357448E-811.53291618
AGGGCGC752.117106E-411.37503710
GCCGGTT1604.7293724E-1111.25682111
AGACCGT600.005945509311.0660286
CGCATTT600.005986349711.05585419
ATTTAGA9000.010.9644511
GGCGAGG14400.010.92423619
CCCGTAC1158.9516107E-710.72677816
TCCAACG1951.8189894E-1210.68801418
CGGTTTC1605.6024874E-1010.67055413
AAATGTC8750.010.6236447
TGTCCAC10050.010.46956310
TAGAAAT9700.010.4627674
AATGTCC8800.010.4191598
CTTACAC2100.010.3882643
GTCCTAC29050.010.3265881
Produced by FastQC (version 0.11.5)