FastQCFastQC Report
Thu 26 May 2016
SRR1512430_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512430_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2077244
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT82500.39716085351552344No Hit
CTGTAGGACGTGGAATATGGCAAGA63050.30352717350489394No Hit
GTCCTACAGTGGACATTTCTAAATT60380.2906736040638461No Hit
GTCCTAAAGTGTGTATTTCTCATTT59770.28773702078330715No Hit
GGTATCAACGCAGAGTACTTTTTTT58240.28037149222720104No Hit
CTTTAGGACGTGAAATATGGCGAGG56550.27223571231882243No Hit
TATCAACGCAGAGTACTTTTTTTTT53160.2559160117925482No Hit
CTGAAGGACCTGGAATATGGCGAGA31100.1497176065979731No Hit
GTCCTACAGTGTGCATTTCTCATTT29530.14215951520379888No Hit
ACGCAGAGTACTTTTTTTTTTTTTT25680.12362534203974113No Hit
ATTTAGAAATGTCCACTGTAGGACG25250.12155529153050869No Hit
TTTCTAAATTTTCCACCTTTTTCAG24150.11625981348363505No Hit
GTCCTTCAGTGTGCATTTCTCATTT24000.11553770284087955No Hit
CTGTAGGACCTGGAATATGGCGAGA23490.11308252665551086No Hit
GAATATGGCAAGAAAACTGAAAATC21550.10374322900920643No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14450.014.1620571
ACCGAGG604.0976537E-412.6649818
GGCGAGG15050.012.42757619
CCACCTT9050.011.43826213
CACCTTT9150.011.41484614
CGTGCGC600.005883665311.08159210
TCCAACG2150.011.03998918
TAGGACC8400.010.9777684
ACCTTTT8700.010.91388215
ATAGGAC2800.010.86459353
AGTCGTC2500.010.6385838
AAGTCGT2600.010.5947447
AGGACGT35600.010.5749235
GGACGTG35100.010.5273426
TTAGGAC22400.010.5250753
TGGCGAG33200.010.40951318
GACGTGG16100.010.3247127
GACGTGA19900.010.2624537
GCCGGTT1409.713767E-810.17697111
TGTAGGA31550.010.1249492
Produced by FastQC (version 0.11.5)