FastQCFastQC Report
Thu 26 May 2016
SRR1512429_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512429_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2025490
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT79520.392596359399454No Hit
GGTATCAACGCAGAGTACTTTTTTT52730.26033206779594076No Hit
TATCAACGCAGAGTACTTTTTTTTT49440.24408908461656192No Hit
GTCCTACAGTGGACATTTCTAAATT44710.22073671062310846No Hit
GTCCTAAAGTGTGTATTTCTCATTT40480.19985287510676428No Hit
CTGTAGGACGTGGAATATGGCAAGA40040.19768056124690814No Hit
CTTTAGGACGTGAAATATGGCGAGG35100.17329140109306884No Hit
GTCCTACAGTGTGCATTTCTCATTT27320.13488094238924903No Hit
ACGCAGAGTACTTTTTTTTTTTTTT23500.11602130842413441No Hit
GTACTTTTTTTTTTTTTTTTTTTTT20980.10357987449950382No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14100.014.350271
TAGGACC10200.012.7390884
GGATAAG1301.4333636E-912.1479221
GTGTAGG3700.012.0042811
AATCCCG2450.011.21661219
CGTGCGC600.00598345111.05655210
GGCGAGG11450.011.00717619
GTCCTAA12650.011.0015521
TGTAGGA30500.010.9464062
GTAGGAC30750.010.4870113
GTCCTAC22200.010.403711
AGGACCT17600.010.4009585
GCGACTA2100.010.39354816
TAAGCGA2100.010.38941813
AATGTCC7300.010.3567588
GCGCAGA1052.695667E-510.3401951
TGGCAAG22850.010.15860818
AAGCGAC2151.8189894E-1210.15209114
GTATCAA30100.010.1325161
GGCAAGA21350.010.11967919
Produced by FastQC (version 0.11.5)