FastQCFastQC Report
Thu 26 May 2016
SRR1512429_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512429_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2025490
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT97040.47909394763736185No Hit
GGTATCAACGCAGAGTACTTTTTTT67870.3350794128828086No Hit
TATCAACGCAGAGTACTTTTTTTTT65850.32510651743528723No Hit
GTCCTACAGTGGACATTTCTAAATT47000.23204261684826882No Hit
CTGTAGGACGTGGAATATGGCAAGA45310.2236989567956396No Hit
GTCCTAAAGTGTGTATTTCTCATTT42320.2089370967025263No Hit
CTTTAGGACGTGAAATATGGCGAGG40880.20182770588845167No Hit
ACGCAGAGTACTTTTTTTTTTTTTT32470.1603068887034742No Hit
GTCCTACAGTGTGCATTTCTCATTT27470.1356215039323818No Hit
GTACTTTTTTTTTTTTTTTTTTTTT24630.12160020538240131No Hit
CTGAAGGACCTGGAATATGGCGAGA21110.10422169450355222No Hit
GAGTACTTTTTTTTTTTTTTTTTTT20980.10357987449950382No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTTGA350.002150640816.3081461
GGTATCA16300.013.7151821
GATCGTC655.4595374E-513.1524447
TAGGACC9600.012.3782564
TCTACGT550.003053314512.0991833
CGTTGGA752.0807517E-411.39625213
GCGTAGC600.00581680111.0985981
TACGCTG600.0058457811.091195
GGCGAGG12700.010.99027719
TAGGACA8650.010.8802724
TTAGGAC17350.010.8489173
GTGTAGG2700.010.5700941
TATGTCG909.568342E-510.55000116
AGGACCT18300.010.5457235
TGGCGAG26650.010.47478618
TGGTATC5250.010.3210892
GACGTGA14900.010.2640077
GCATAAT1303.7704558E-710.244861
TGTAGGA27050.010.0861312
GGTATAC856.511852E-410.0726781
Produced by FastQC (version 0.11.5)