FastQCFastQC Report
Thu 26 May 2016
SRR1512428_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512428_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1681826
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT64020.3806576899156036No Hit
GTCCTAAAGTGTGTATTTCTCATTT58530.34801459841862353No Hit
GTCCTACAGTGGACATTTCTAAATT57110.33957139442486917No Hit
CTGTAGGACGTGGAATATGGCAAGA54820.3259552415053638No Hit
CTTTAGGACGTGAAATATGGCGAGG49680.29539322141529506No Hit
GGTATCAACGCAGAGTACTTTTTTT42450.2524042320668131No Hit
TATCAACGCAGAGTACTTTTTTTTT38830.2308800078010448No Hit
GTCCTACAGTGTGCATTTCTCATTT32460.19300450819525922No Hit
CTGAAGGACCTGGAATATGGCGAGA24390.14502094747019012No Hit
GTCCTTCAGTGTGCATTTCTCATTT22700.13497234553396129No Hit
ATTTAGAAATGTCCACTGTAGGACG22340.1328318149439954No Hit
CTGTAGGACCTGGAATATGGCGAGA21920.13033452925570183No Hit
TTTCTAAATTTTCCACCTTTTTCAG19780.11761026408201561No Hit
GAATATGGCAAGAAAACTGAAAATC19760.1174913457159064No Hit
ACGCAGAGTACTTTTTTTTTTTTTT19020.11309136616986537No Hit
GTACTTTTTTTTTTTTTTTTTTTTT17530.10423194789472871No Hit
GGAATATGGCGAGAAAACTGAAAAT17050.10137790710810751No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11050.013.9398361
TAGGACC9750.013.7157364
CCGTGCA500.001550910613.240789
GTGTAGG3550.012.7945441
ACCTTTT8400.011.85996715
TAGAAAT8800.011.8553564
CCACCTT8400.011.51835413
AGGACCT18500.011.2273445
GGCGAGG15450.011.16441419
GGACCTG17900.011.1264826
ATGTCCA9200.011.1025179
TTAGGAC21650.011.0394453
AAATGTC9300.011.01367957
GAAATGT9400.010.9974066
ATCCTAC2001.8189894E-1210.8614551
AATGTCC9550.010.788868
TATGTCG1502.1809683E-910.75207216
CACCTTT9250.010.6675614
GCCGGTT803.8471445E-410.664411
GCCTAAG1302.2582208E-710.6335931
Produced by FastQC (version 0.11.5)